一种乙酰胆碱生物传感器，其通过将乙酰胆碱酯酶和聚乙二醇修饰的胆碱氧化酶包埋在聚乙烯醇冷冻凝胶膜中制成。

Acetylcholine biosensor involving entrapment of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane.

作者信息

Doretti L, Ferrara D, Lora S, Schiavon F, Veronese FM

机构信息

Servizio di Prevenzione e Protezione del Consiglio Nazionale delle Ricerche, Corso Stati Uniti 4, 35127, Padova, Italy

出版信息

Enzyme Microb Technol. 2000 Aug 1;27(3-5):279-285. doi: 10.1016/s0141-0229(00)00210-6.

DOI:10.1016/s0141-0229(00)00210-6

PMID:10899554

Abstract

A bienzymatic sensor for the determination of acetylcholine was prepared by physical coimmobilization of acetylcholinesterase and poly(ethylene glycol)-modified choline oxidase in a poly(vinyl alcohol) cryogel membrane obtained by a cyclic freezing-thawing process. The enzyme-modified polymer was applied on a platinum electrode to form an amperometric sensor, based on the electrochemical detection of enzymatically developed hydrogen peroxide. The analytical characteristics of this sensor, including calibration curves for choline and acetylcholine, pH, and temperature effects, and stability are described.

摘要

通过循环冻融法制备的聚乙烯醇冷冻凝胶膜，将乙酰胆碱酯酶和聚乙二醇修饰的胆碱氧化酶物理共固定化，制备了一种用于测定乙酰胆碱的双酶传感器。基于酶促产生的过氧化氢的电化学检测，将酶修饰的聚合物应用于铂电极上，形成电流型传感器。描述了该传感器的分析特性，包括胆碱和乙酰胆碱的校准曲线、pH和温度影响以及稳定性。