一种编码人CTP合成酶同工型的cDNA的鉴定。

Identification of a cDNA encoding an isoform of human CTP synthetase.

作者信息

van Kuilenburg A B, Meinsma R, Vreken P, Waterham H R, van Gennip A H

机构信息

Academic Medical Center, University of Amsterdam, Emma Children's Hospital and Department of Clinical Chemistry, Laboratory Genetic Metabolic Diseases, PO Box 22700, 1100 DE, Amsterdam, The Netherlands.

出版信息

Biochim Biophys Acta. 2000 Jul 24;1492(2-3):548-52. doi: 10.1016/s0167-4781(00)00141-x.

DOI:10.1016/s0167-4781(00)00141-x

PMID:10899599

Abstract

A full-length cDNA clone encoding an isoform of human CTP synthetase (type II) was isolated. A 1761-nucleotide open reading frame which corresponds to a protein of 586 amino acids with a predicted molecular mass of 65678 Da was identified. The predicted protein sequence showed 74% identity with the translation product of a previously identified human CTP synthetase cDNA clone (type I). The function of the human cDNA encoding type II CTP synthetase was verified by successful complementation of the cytidine-requiring CTP synthetase deficient mutant JF618 of Escherichia coli. The gene encoding type II CTP synthetase has been localized on chromosome Xp22.

摘要

分离出了一个编码人CTP合成酶（II型）同工型的全长cDNA克隆。鉴定出一个1761个核苷酸的开放阅读框，它对应于一个由586个氨基酸组成的蛋白质，预测分子量为65678道尔顿。预测的蛋白质序列与先前鉴定的人CTP合成酶cDNA克隆（I型）的翻译产物具有74%的同一性。通过成功互补大肠杆菌需要胞苷的CTP合成酶缺陷型突变体JF618，验证了编码II型CTP合成酶的人cDNA的功能。编码II型CTP合成酶的基因已定位在Xp22染色体上。