Zhai C, Skaanild M T, Chu E H
Laboratory of Molecular Biology, Hong Kong University of Science and Technology.
Hua Xi Yi Ke Da Xue Xue Bao. 1995 Mar;26(1):45-9.
The CTP synthetase cDNA was cloned from Chinese hamster lung cells V79. By comparison of the amino acid sequence of CTP synthetase with the counterpart of other species published, it was show that the CTP synthetase was a highly conserved and stable gene among the mammalian cells, but more differences of the CTP synthetase sequence could be seen between procaryotic and eukaryotic cells. This phenomenon suggested that the catalysing model of the enzyme might have some small differences in the organisms.
CTP合成酶cDNA是从中国仓鼠肺细胞V79中克隆出来的。通过将CTP合成酶的氨基酸序列与已发表的其他物种的对应序列进行比较,发现CTP合成酶在哺乳动物细胞中是一个高度保守且稳定的基因,但在原核细胞和真核细胞之间可以看到CTP合成酶序列有更多差异。这种现象表明该酶的催化模式在不同生物中可能存在一些细微差异。