Lai J Y, Huang C F, Tseng Y H, Yang M T
Institute of Molecular Biology, National Chung Hsing University, 402, Taichung, Taiwan.
Biochim Biophys Acta. 2000 Jul 24;1492(2-3):553-9. doi: 10.1016/s0167-4781(00)00143-3.
The Xanthomonas campestris rpsM (S13)-rpsK (S11)-rpsD (S4)-rpoA (alpha)-rplQ (L17) cluster, encoding RNA polymerase alpha-subunit and four ribosomal proteins, reside in a 3164-bp DNA region. The N-terminal sequence of the authentic alpha-protein determined chemically matches that predicted from the nucleotide sequence. rplQ is monocistronic, instead of being co-transcribed with the other genes as in Escherichia coli. Antiserum against the His-tagged alpha-protein cross-reacted with the E. coli alpha-protein.
野油菜黄单胞菌的rpsM（S13）-rpsK（S11）-rpsD（S4）-rpoA（α）-rplQ（L17）基因簇编码RNA聚合酶α亚基和四种核糖体蛋白，位于一个3164 bp的DNA区域。化学测定的真实α蛋白的N端序列与核苷酸序列预测的序列相符。rplQ是单顺反子，不像在大肠杆菌中那样与其他基因共转录。抗His标签α蛋白的抗血清与大肠杆菌α蛋白发生交叉反应。
