Meek D W, Hayward R S
Nucleic Acids Res. 1984 Jul 25;12(14):5813-21. doi: 10.1093/nar/12.14.5813.
The "alpha-operon" of E.coli is a unit of regulation comprising the following known genes, mostly encoding ribosomal proteins (in order of transcription, and with their products named in brackets): rpsM (S13), rpsK (S11), rpsD (S4), rpoA (alpha-subunit of RNA polymerase), rplQ (L17). There is evidence that S4 tightly regulates all of these genes, except rpoA, by repressing translation of the polycistronic mRNA. Binding of S4 to the S13 start-site is thought to regulate the first three genes. We have extended the 'rpsD-rpoA' sequences previously determined by others, to include all of rpoA and rplQ. The rpoA-rplQ intercistronic region shows strong primary, and potential secondary structural homologies with the S4-binding sites on 16S rRNA and S13 mRNA. We suggest that S4 represses L17 translation directly.
大肠杆菌的“α操纵子”是一个调控单位,由以下已知基因组成,这些基因大多编码核糖体蛋白(按转录顺序排列,括号内为其产物名称):rpsM(S13)、rpsK(S11)、rpsD(S4)、rpoA(RNA聚合酶的α亚基)、rplQ(L17)。有证据表明,S4通过抑制多顺反子mRNA的翻译,紧密调控除rpoA之外的所有这些基因。S4与S13起始位点的结合被认为可调控前三个基因。我们将其他人先前测定的“rpsD - rpoA”序列进行了扩展,纳入了整个rpoA和rplQ。rpoA - rplQ基因间区域与16S rRNA和S13 mRNA上的S4结合位点表现出很强的一级结构以及潜在的二级结构同源性。我们认为S4直接抑制L17的翻译。