Popov Z, Gil-Diez de Medina S, Lefrere-Belda M A, Hoznek A, Bastuji-Garin S, Abbou C C, Thiery J P, Radvanyi F, Chopin D K
Centre de Recherches Chirurgicales, EMI-INSERM 99-09 Département de Santé Publique et de Pathologie, Hôpital Henri Mondor, Créteil, France.
Br J Cancer. 2000 Jul;83(2):209-14. doi: 10.1054/bjoc.2000.1233.
We studied E-cadherin down-regulation at the protein level in frozen sections of 111 bladder tumours and 13 normal bladder specimens by means of immunohistochemistry, and at the mRNA level by semi-quantitative RT-PCR in 40 of the same tumours. Results indicate that E-cadherin expression detected by immunohistochemistry correlated with both stage and grade (P < 0.0001 and P < 0.001, respectively). Analysis of recurrence, progression and survival over a mean period of 36 months after surgery in the entire cohort showed that abnormal E-cadherin immunoreactivity correlated strongly with poor outcome (log-rank test: P = 0.001, P = 0.0001 and P = 0.0003, respectively). In multistep logistic regression analysis, only E-cadherin status and stage had significant additional prognostic value (P= 0.008 and OR = 0.2; P= 0.03 and OR = 3.6, respectively). Survival estimates derived from RT-PCR transcript quantification differed significantly for low and high expression (log-rank test: P = 0.0006). These results suggest that the alteration occurs at the transcriptional level and support the clinical and biological relevance of cell adhesion molecules in bladder cancer.
我们通过免疫组织化学方法，在111例膀胱肿瘤和13例正常膀胱标本的冰冻切片上研究了E-钙黏蛋白在蛋白质水平的下调情况，并通过半定量逆转录聚合酶链反应（RT-PCR）在其中40例相同肿瘤中研究了其在mRNA水平的情况。结果表明，免疫组织化学检测到的E-钙黏蛋白表达与分期和分级均相关（分别为P < 0.0001和P < 0.001）。对整个队列术后平均36个月的复发、进展和生存情况进行分析显示，异常的E-钙黏蛋白免疫反应性与不良预后密切相关（对数秩检验：分别为P = 0.001、P = 0.0001和P = 0.0003）。在多步逻辑回归分析中，只有E-钙黏蛋白状态和分期具有显著的额外预后价值（分别为P = 0.008，比值比[OR] = 0.2；P = 0.03，OR = 3.6）。通过RT-PCR转录本定量得出的低表达和高表达生存估计值有显著差异（对数秩检验：P = 0.0006）。这些结果表明这种改变发生在转录水平，并支持细胞黏附分子在膀胱癌中的临床和生物学相关性。
