Ruijter E T, Miller G J, Aalders T W, van de Kaa C A, Schalken J A, Debruyne F M, Boon M E
Department of Urology, University Hospital Nijmegen, The Netherlands.
J Pathol. 1997 Nov;183(3):369-75. doi: 10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-9.
Conventional fixation of large solid surgical specimens is a slow process. Consequently, autolytic damage to tissues may occur if the fixative does not reach the central part of the specimen in time. However, as there is also a time relationship between formalin fixation and antigen masking, fixation for too long can also be detrimental. In seeking the optimum balance for fixation, microwave irradiation might be of assistance. This study set out to evaluate methods for fixing entire prostate glands within a brief period of time, using microwave-stimulated formalin fixation. The results show that entire prostates can be optimally fixed if formalin is present throughout the tissue as the temperature is increased by microwave irradiation. This is achieved by injecting the fixative into the prostate at multiple sites immediately following prostatectomy. The technique described ensures standardization of a critical step during tissue processing, leading to uniform microscopic results with both routine and immunohistochemical stains. It is a simple, rapid method, suitable for routine diagnostic use. Using this modified approach, DNA of much larger sizes can be extracted from paraffin-embedded material, which could expand the possibilities for molecular analysis.
大型实体手术标本的传统固定是一个缓慢的过程。因此，如果固定剂不能及时到达标本的中心部分，可能会发生组织自溶损伤。然而，由于福尔马林固定与抗原掩盖之间也存在时间关系，固定时间过长也可能有害。在寻求固定的最佳平衡时，微波辐射可能会有所帮助。本研究旨在评估使用微波刺激福尔马林固定在短时间内固定整个前列腺的方法。结果表明，如果在微波辐射使温度升高的同时，整个组织中都存在福尔马林，那么整个前列腺可以得到最佳固定。这是通过在前列腺切除术后立即在多个部位将固定剂注入前列腺来实现的。所描述的技术确保了组织处理过程中关键步骤的标准化，从而在常规染色和免疫组织化学染色中产生一致的微观结果。这是一种简单、快速的方法，适用于常规诊断用途。使用这种改进方法，可以从石蜡包埋材料中提取更大尺寸的DNA，这可能会扩大分子分析的可能性。
