Deutsch J, Kalderon B, Purdon A D, Rapoport S I
Department of Medicinal Chemistry, Faculty of Medicine, The Hebrew University of Jerusalem, Israel.
Lipids. 2000 Jun;35(6):693-6. doi: 10.1007/s11745-000-0575-1.
Concentration and distribution of long-chain acylcarnitines in control microwaved and ischemic (decapitated) rat brain were measured by electrospray ionization tandem mass spectrometry. The total acylcarnitine concentration from control rat brains equaled 7-8 nmol/g wet weight brain, about one-fourth the total concentration of long-chain acyl-CoA, indicating a small role in buffering the total acyl-CoA pool concentration. Furthermore, acylcarnitine did not differ between ischemic and control rat brain with regard to total concentration or concentrations of molecular species of acylcarnitine. Therefore, the size of the acylcarnitine pool in brain is not affected by the dramatic increase in unesterified fatty acids (approximately 4x) that occurs in ischemia.
通过电喷雾电离串联质谱法测量了对照微波处理和缺血（断头）大鼠脑中长链酰基肉碱的浓度和分布。对照大鼠脑的总酰基肉碱浓度等于7-8 nmol/g湿重脑，约为长链酰基辅酶A总浓度的四分之一，表明其在缓冲总酰基辅酶A池浓度方面作用较小。此外，缺血大鼠脑和对照大鼠脑之间的酰基肉碱在总浓度或酰基肉碱分子种类浓度方面没有差异。因此，脑内酰基肉碱池的大小不受缺血时未酯化脂肪酸显著增加（约4倍）的影响。
