Yu Y, Rabinowitz R, Polliack A, Ben-Bassat H, Schlesinger M
Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
Eur J Haematol. 2000 Jun;64(6):376-84. doi: 10.1034/j.1600-0609.2000.90144.x.
In the present study the cell surface expression of CD45 isoforms on normal and neoplastic human B cells was correlated with splice products of the CD45 mRNA, using RT-PCR technology. In non-Hodgkin's lymphoma cells in the leukemic phase (NHL) the majority of the cells expressed a high level of CD45RA, while in CLL most of the cells expressed a low level. In the Raji and Daudi Burkitt B-cell lymphoma lines the main CD45 mRNA product was the largest, unspliced, full-length isoform (456) and the 56 splice product. Similar results were obtained with B-cell lymphoma cells isolated from the peripheral blood of patients with NHL in the leukemic phase. In EBV-transformed B-cell lines, the 456 and the 56 isoform of CD45 mRNA were predominant, but in addition a low level of the 5- and 0-exon splice products was detected. A strikingly different pattern was obtained with B-CLL cells. In CLL the level of the 456 and the 56 isoforms was low, while that of the 5- and 0-exon splice products was increased. Thus, in contrast to the heterogeneity in the expression of CD45RO in B-CLL, the majority of the cells contained the CD45 mRNA splice product coding for CD45RO. Analysis of splice products of the CD45 mRNA may serve as an additional tool to differentiate CLL from the leukemic phase of NHL.
在本研究中，运用逆转录聚合酶链反应（RT-PCR）技术，将正常和肿瘤性人类B细胞上CD45亚型的细胞表面表达与CD45 mRNA的剪接产物进行关联分析。在白血病期的非霍奇金淋巴瘤（NHL）细胞中，大多数细胞高水平表达CD45RA，而在慢性淋巴细胞白血病（CLL）中，大多数细胞低水平表达。在拉吉（Raji）和道迪（Daudi）伯基特B细胞淋巴瘤细胞系中，主要的CD45 mRNA产物是最大的、未剪接的全长亚型（456）和56剪接产物。从处于白血病期的NHL患者外周血中分离出的B细胞淋巴瘤细胞也得到了类似结果。在EB病毒转化的B细胞系中，CD45 mRNA的456和56亚型占主导，但此外还检测到低水平的5-和0-外显子剪接产物。B-CLL细胞呈现出截然不同的模式。在CLL中，456和56亚型的水平较低，而5-和0-外显子剪接产物的水平升高。因此，与B-CLL中CD45RO表达的异质性相反，大多数细胞含有编码CD45RO的CD45 mRNA剪接产物。分析CD45 mRNA的剪接产物可能成为区分CLL与NHL白血病期的额外工具。
