Huang C C, Novak W R, Babbitt P C, Jewett A I, Ferrin T E, Klein T E
Department of Pharmaceutical Chemistry, University of California, San Francisco 94143-0446, USA.
Pac Symp Biocomput. 2000:230-41. doi: 10.1142/9789814447331_0022.
We have developed new computational methods for displaying and analyzing members of protein superfamilies. These methods (MinRMS, AlignPlot and MSFviewer) integrate sequence and structural information and are implemented as separate but cooperating programs to our Chimera molecular modeling system. Integration of multiple sequence alignment information and three-dimensional structural representations enable researchers to generate hypotheses about the sequence-structure relationship. Structural superpositions can be generated and easily tuned to identify similarities around important characteristics such as active sites or ligand binding sites. Information related to the release of Chimera, MinRMS, AlignPlot and MSFviewer can be obtained at http:¿www.cgl.ucsf.edu/chimera.
我们已经开发出了用于展示和分析蛋白质超家族成员的新计算方法。这些方法（MinRMS、AlignPlot和MSFviewer）整合了序列和结构信息，并作为独立但相互协作的程序集成到我们的Chimera分子建模系统中。多序列比对信息与三维结构表示的整合使研究人员能够生成关于序列-结构关系的假设。可以生成结构叠加并轻松调整，以识别重要特征（如活性位点或配体结合位点）周围的相似性。有关Chimera、MinRMS、AlignPlot和MSFviewer发布的信息可在http://www.cgl.ucsf.edu/chimera获取。
