Prystowsky M B, Sorokin C F, Ceglowski W S, Hirschhorn K, Glade P R
Int Arch Allergy Appl Immunol. 1975;48(2):225-35. doi: 10.1159/000231309.
Subcellular fractions of the human lymphoid cell line PGLC-33H were obtained by N2 cavitation and differential centrifugation. The purity of the fractions was assessed by the use of the following marker enzymes: beta-glucuronidase for the lysosomal-intermediate fraction; a nonspecific esterase for the microsomal fraction; and LDH for the supernatant fraction. These subcellular fractions were studied for MIF activity utilizing human lymphoid cells from established lines as target cells. MIF activity was most consistently found in the microsomal fraction. Also, MIF activity was closely associated with the relative specific activity of exterase. No such correlation with MIF activity could be demonstrated for beta-glucuronidase or LDH.
通过氮气空化和差速离心法获得人淋巴母细胞系PGLC - 33H的亚细胞组分。使用以下标记酶评估各组分的纯度：溶酶体 - 中间组分用β - 葡萄糖醛酸酶；微粒体组分用非特异性酯酶；上清液组分用乳酸脱氢酶。利用已建立细胞系的人淋巴细胞作为靶细胞，对这些亚细胞组分进行迁移抑制因子（MIF）活性研究。在微粒体组分中最一致地发现了MIF活性。此外，MIF活性与酯酶的相对比活性密切相关。对于β - 葡萄糖醛酸酶或乳酸脱氢酶，未发现与MIF活性有这种相关性。
