Miks B, Kawiak J, Hoser G
Folia Histochem Cytochem (Krakow). 1978;16(3):187-92.
The microsomal fraction was isolated from homogenate of 125I-labeled leukemia L 1210 ascites cells by filtration of postmitochondrial supernatant through a Sepharose 4 B column. It was found that the particles are labeled with iodine and show 5'-nucleotidase activity suggesting the presence of cell membranes in the fraction. The soluble proteins fraction were retarded on the column showed lactate dehydrogenase activity, and low activity of soluble beta-D-glucuronidase.
通过将线粒体后上清液经琼脂糖4B柱过滤，从125I标记的白血病L 1210腹水细胞匀浆中分离出微粒体部分。发现这些颗粒被碘标记，并显示5'-核苷酸酶活性，表明该部分存在细胞膜。在柱上滞留的可溶性蛋白质部分显示乳酸脱氢酶活性和低活性的可溶性β-D-葡萄糖醛酸酶。
