Ramírez-Romero R, Brogden K A, Gallup J M, Dixon R A, Ackermann M R
Department of Veterinary Pathology, Iowa State University, Ames, Iowa 50011-1250, USA.
J Comp Pathol. 2000 Jul;123(1):29-35. doi: 10.1053/jcpa.1999.0383.
Mast cells in the left cranial pulmonary lobe of colostrum-deprived neonatal calves were quantified 2 and 6 h after intrabronchial inoculation with Mannheimia (Pasteurella) haemolytica A1. The mast cells were detected (1) immunohistochemically with a mouse anti-human mast cell tryptase monoclonal antibody, and (2) by metachromatic staining with low pH toluidine blue. A greater number of mast cells was demonstrated by the second method than by the first. At 6 h after inoculation, but not at 2 h, the number of mast cells was significantly reduced at the site of the main lesions. Treatment of calves with a sialyl Lewis mimetic (TBC1269) did not appreciably affect the results at 6 h.
