Pace L W, Turk J R, Corstvet R E, Enright F M, Henk W
Department of Veterinary Pathology, Louisiana State University, Baton Rouge 70803.
Can J Vet Res. 1994 Apr;58(2):79-82.
Dose dependent pulmonary lesions of acute bronchopneumonia were induced in male, outbred Swiss Webster mice by intrabronchial inoculation of Pasteurella haemolytica. Five exponential dilutions ranging from 5 x 10(4) to 5 x 10(8) colony forming units per mL (CFU/mL) of Pasteurella haemolytica serotype 1 were inoculated into five groups of mice. Mice were killed by cervical dislocation 24 hours postinoculation. Pulmonary lesions occurred in mice of all five groups, however, 5 x 10(7) CFU/mL was the minimal dose which consistently produced lesions. Focal parenchymal necrosis, suppurative bronchiolitis, and flooding of interalveolar septa and alveoli by edema fluid, fibrin, neutrophils and macrophages, were observed microscopically. We conclude that outbred Swiss Webster mice can be used as a model for the study of selected disease mechanisms of acute lung inflammation and that this model may be used to determine some of the pathogenic mechanisms involved in the development of pulmonary lesions in bovine pneumonic pasteurellosis.
通过向雄性远交群瑞士韦伯斯特小鼠支气管内接种溶血巴斯德菌，诱导出剂量依赖性急性支气管肺炎的肺部病变。将每毫升含有5×10⁴至5×10⁸菌落形成单位（CFU/mL）的1型溶血巴斯德菌的五个指数稀释液接种到五组小鼠中。接种后24小时通过颈椎脱臼处死小鼠。所有五组小鼠均出现肺部病变，然而，5×10⁷CFU/mL是始终能产生病变的最小剂量。显微镜下观察到局灶性实质坏死、化脓性细支气管炎以及肺泡间隔和肺泡被水肿液、纤维蛋白、中性粒细胞和巨噬细胞充满。我们得出结论，远交群瑞士韦伯斯特小鼠可作为研究急性肺部炎症特定疾病机制的模型，并且该模型可用于确定牛肺炎巴氏杆菌病肺部病变发展过程中涉及的一些致病机制。
