Braat A K, van de Water S, Goos H, Bogerd J, Zivkovic D
Department of Experimental Zoology, Utrecht University, Padualaan 8, 3584 CH, The, Utrecht, Netherlands.
Mech Dev. 2000 Jul;95(1-2):271-4. doi: 10.1016/s0925-4773(00)00344-0.
Primordial germ cell (PGC) development in the zebrafish is poorly understood. The expression of vasa RNA, the only molecular marker so far found to be expressed in fish PGCs, suggests its function in the establishment of the germline. The protein product of vasa is present throughout the life cycle in the germline of Drosophila, Caenorhabditis and Xenopus. The expression pattern of the Vasa protein in zebrafish, is still unresolved. We generated an anti-Vasa polyclonal antibody and show that it is maternally expressed initially throughout the embryo. Interestingly, from the two-cell- to the 1000-cell stage the protein is highly concentrated in two 'dots' near the center of the blastomeres and as such remains longest detectable in the animal pole blastomeres. The first distinct cell-specific expression occurs at 60% epiboly on one side of the blastoderm margin. The Vasa protein in the PGCs is organized in a subcellular granular-like conformation which is dynamic throughout development.
斑马鱼原始生殖细胞（PGC）的发育过程目前还了解甚少。瓦萨RNA（vasa RNA）是迄今为止发现的唯一在鱼类PGC中表达的分子标记，其表达表明了它在生殖系建立过程中的作用。瓦萨蛋白产物在果蝇、秀丽隐杆线虫和非洲爪蟾的整个生命周期中都存在于生殖系中。斑马鱼中瓦萨蛋白的表达模式仍未明确。我们制备了一种抗瓦萨多克隆抗体，并发现它最初在整个胚胎中由母体表达。有趣的是，从二细胞期到1000细胞期，该蛋白高度集中在卵裂球中心附近的两个“点”上，因此在动物极卵裂球中可检测到的时间最长。首次明显的细胞特异性表达出现在胚盘边缘一侧的60%外包期。PGC中的瓦萨蛋白以亚细胞颗粒状构象存在，且在整个发育过程中都是动态变化的。
