State Key Laboratory of Biocontrol, Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals, Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China.
Int J Mol Sci. 2021 Nov 22;22(22):12607. doi: 10.3390/ijms222212607.
Spermatogenesis is a process of self-renewal and differentiation in spermatogonial stem cells. During this process, germ cells and somatic cells interact intricately to ensure long-term fertility and accurate genome propagation. Spermatogenesis has been intensely investigated in mammals but remains poorly understood with regard to teleosts. Here, we performed single-cell RNA sequencing of ~9500 testicular cells from the male, orange-spotted grouper. In the adult testis, we divided the cells into nine clusters and defined ten cell types, as compared with human testis data, including cell populations with characteristics of male germ cells and somatic cells, each of which expressed specific marker genes. We also identified and profiled the expression patterns of four marker genes (, , , ) in both the ovary and adult testis. Our data provide a blueprint of male germ cells and supporting somatic cells. Moreover, the cell markers are candidates that could be used for further cell identification.
精子发生是精原干细胞自我更新和分化的过程。在此过程中，生殖细胞和体细胞相互作用错综复杂，以确保长期的生育能力和基因组的准确传播。精子发生在哺乳动物中已被深入研究，但对于鱼类仍知之甚少。在这里，我们对雄性橙色斑点石斑鱼的约 9500 个睾丸细胞进行了单细胞 RNA 测序。在成年睾丸中，我们将细胞分为九个簇，并定义了十种细胞类型，与人类睾丸数据相比，包括具有雄性生殖细胞和体细胞特征的细胞群体，每个群体都表达了特定的标记基因。我们还鉴定并分析了四个标记基因（ 、 、 、 ）在卵巢和成年睾丸中的表达模式。我们的数据为雄性生殖细胞和支持体细胞提供了一个蓝图。此外，这些细胞标记物是可用于进一步细胞鉴定的候选物。
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