Banks K L, McGuire T C
J Immunol. 1975 Apr;114(4):1307-13.
Studies were conducted to determine the mechanisms of antibody suppression of phytohemagglutinin (PHA)- and concanavalin A (Con A)-induced lymphocyte proliferation. Initial experiments incubated lymphocytes with either PHA or Con A, and at various times the respective anti-phytolectin was added to the cultures. DNA synthesis was less than 20% of the total response if anti-PHA was added within 2 hr and if anti-Con A was added within 10 hr of addition of mitogens to the culture. At the concentrations used, anti-PHA reduced PHA binding to the lymphocytes, while anti-Con A did not reduce Con A binding to the cells. Antibody was non-toxic and specific for its respective mitogen. Additional experiments incubated lymphocytes with either PHA or Con A and then the cells were washed and placed in mitogen-free media. The addition of antibody to cultures which had been washed markedly suppressed proliferation. Using radiolabeled PHA and Con A in one group of experiments and fluorescent labeled anti-mitogen antibodies in others, it was determined that antibody did not remove Con A or PHA from the cell, but instead antibody slowed the release of mitogen from the lymphocyte. Anti-mitogen antibody remained attached to mitogen on the surface of lymphocytes for at least 24 hr. The experiments suggest that antibody can suppress the lymphocyte reaction by blocking the necessary phytolectinlymphocyte interaction and interrupting stimulation by cell-bound mitogen.
开展了多项研究以确定抗体抑制植物血凝素（PHA）和刀豆球蛋白A（Con A）诱导的淋巴细胞增殖的机制。最初的实验将淋巴细胞与PHA或Con A一起孵育，并在不同时间向培养物中加入相应的抗植物凝集素。如果在向培养物中加入促细胞分裂剂后的2小时内加入抗PHA，或者在10小时内加入抗Con A，DNA合成量不到总反应的20%。在所使用的浓度下，抗PHA降低了PHA与淋巴细胞的结合，而抗Con A并未降低Con A与细胞的结合。抗体对其相应的促细胞分裂剂无毒且具有特异性。另外的实验将淋巴细胞与PHA或Con A一起孵育，然后洗涤细胞并将其置于无促细胞分裂剂的培养基中。向已洗涤过的培养物中加入抗体可显著抑制增殖。在一组实验中使用放射性标记的PHA和Con A，在其他实验中使用荧光标记的抗促细胞分裂剂抗体，结果确定抗体并未从细胞中去除Con A或PHA，而是减缓了促细胞分裂剂从淋巴细胞中的释放。抗促细胞分裂剂抗体在淋巴细胞表面与促细胞分裂剂结合至少24小时。这些实验表明，抗体可通过阻断必要的植物凝集素-淋巴细胞相互作用并中断细胞结合的促细胞分裂剂的刺激来抑制淋巴细胞反应。
