Henkart P A, Fisher R I
J Immunol. 1975 Feb;114(2 Pt 1):710-4.
Human peripheral blood lymphocytes were iodinated by the lactoperoxidase technique, and the surface receptors for the mitogens concanavalin A (Con A) and phytohemagglutinin (PHA) were analyzed by solubilizing the membrane protein and incubating with mitogen and antimitigen antibody. The resulting precipitates were washed and analyzed by gel electrophoresis. Con A was to bind to about twice as much labeled protein as PHA. Sodium dodecyl sulfate gels showed that multiple surface membrane proteins bind to these mitogens and that the pattern of peaks of Con A receptors in generally similar to the pattern of PHA receptors. Long-term lymphoid tissue culture cells lines also show multiple receptor peaks for mitogens, indicating that the heterogeneity of receptors is not caused entirely by lymphocyte heterogeneity.
采用乳过氧化物酶技术对人外周血淋巴细胞进行碘化处理，通过溶解膜蛋白并与促细胞分裂剂及抗促细胞分裂剂抗体孵育，分析促细胞分裂剂刀豆球蛋白A（Con A）和植物血凝素（PHA）的表面受体。将所得沉淀物洗涤后进行凝胶电泳分析。Con A结合的标记蛋白量约为PHA的两倍。十二烷基硫酸钠凝胶显示，多种表面膜蛋白与这些促细胞分裂剂结合，且Con A受体的峰型模式通常与PHA受体的峰型模式相似。长期淋巴细胞组织培养细胞系对促细胞分裂剂也显示出多个受体峰，这表明受体的异质性并非完全由淋巴细胞的异质性所致。
