Biberfield P, Ghetie V, Sjöquist J
J Immunol Methods. 1975 Jan;6(3):249-59. doi: 10.1016/0022-1759(75)90068-x.
Staphylococcal protein A (SpA) labeled with FITC was used to detect IgG antibodies to tissue and cell surface antigens. Radioiodinated SpA was employed in a radioimmuno technique to assay IgG antibodies which had reacted with cell membrane antigens. Red blood cells coated with SpA were used as an indicator system in the mixed haemadsorption technique to detect IgG antibodies on tissue culture cells. The results showed that SpA was highly specific in the various systems tested, although it was not as sensitive in the immunofluorescence test as FITC anti-IgG. It was confirmed that SpA reacts with IgG antibodies of human, monkey, rabbit, sheep and mice origin. The reactivity with human and monkey IgG was stronger than with IgG of the other species. We consider labeled SpA as an alternative to monospecific anti-IgG for detection and assaying of IgG antibodies to tissue and cell surface antigens.
用异硫氰酸荧光素（FITC）标记的葡萄球菌蛋白A（SpA）来检测针对组织和细胞表面抗原的IgG抗体。放射性碘化的SpA被用于放射免疫技术中，以测定与细胞膜抗原发生反应的IgG抗体。用包被有SpA的红细胞作为混合血细胞吸附技术中的指示系统，来检测组织培养细胞上的IgG抗体。结果表明，SpA在各种测试系统中具有高度特异性，尽管它在免疫荧光试验中不如FITC抗IgG敏感。已证实SpA能与人、猴、兔、绵羊和小鼠来源的IgG抗体发生反应。与人和猴IgG的反应性强于与其他物种IgG的反应性。我们认为标记的SpA可作为单特异性抗IgG的替代物，用于检测和测定针对组织和细胞表面抗原的IgG抗体。
