Petts V, Roitt I M
Clin Exp Immunol. 1971 Sep;9(3):407-18.
Conjugates of antibody with the enzyme marker, peroxidase, are relatively easy to prepare and can be shown to have a sensitivity in the indirect test for antinuclear antibodies which is entirely comparable to that obtained with fluorescein labelled material. Clear cut results were obtained in tests for autoantibodies on tissue sections and for bound IgG in biopsies using an antihuman immunoglobulin conjugate. Antibodies to were also readily demonstrable. Only a simple light microscope is required, slides can be read without fatigue and the morphology of the tissue section can be easily assessed. It is concluded that the peroxidase technique offers a useful alternative to immunofluorescence.
抗体与酶标记物过氧化物酶的结合物相对容易制备，并且在抗核抗体的间接检测中显示出与荧光素标记材料相当的灵敏度。使用抗人免疫球蛋白结合物对组织切片上的自身抗体和活检中的结合IgG进行检测时，获得了明确的结果。针对……的抗体也很容易检测到。只需要一台简单的光学显微镜，玻片可以轻松读取而不会使人疲劳，并且可以很容易地评估组织切片的形态。结论是，过氧化物酶技术为免疫荧光提供了一种有用的替代方法。
