火箭免疫电泳在传染性法氏囊病诊断中的应用

Rocket immunoelectrophoresis in the diagnosis of infectious bursal disease.

作者信息

Raj G D, Thangavelu A, Elankumaran S, Koteeswaran A

机构信息

Vaccine Research Centre, Tamilnadu Veterinary and Animal Sciences University, Madhavaram Milk Colony, Chennai, India.

出版信息

Trop Anim Health Prod. 2000 Jun;32(3):173-8. doi: 10.1023/a:1005287732214.

DOI:10.1023/a:1005287732214

PMID:10907288

Abstract

The rocket immunoelectrophoresis (RIE) test was used for the qualitative detection and quantitative estimation of infectious bursal disease virus (IBDV) specific antigen in experimentally infected chickens and samples collected from suspected outbreaks. The IBDV specific antigen was detected in the bursae of experimentally inoculated chickens up to 5 days post infection (PI) by the agar gel precipitation (AGP) test and 7 days PI by the RIE test. The RIE detected IBDV specific antigen in a significantly greater number of samples collected from the field outbreaks than the conventional AGP test. Exudative bursae were found to have a higher antigen content than haemorrhagic bursae and are recommended as the material of choice for diagnosis of IBD. This test could also be used to quantify IBDV specific antigen in commercial killed vaccines.

摘要

火箭免疫电泳（RIE）试验用于对实验感染鸡以及从疑似疫情中采集的样本中的传染性法氏囊病病毒（IBDV）特异性抗原进行定性检测和定量评估。通过琼脂凝胶沉淀（AGP）试验，在感染后（PI）长达5天的实验接种鸡的法氏囊中检测到IBDV特异性抗原，而通过RIE试验在感染后7天检测到该抗原。与传统的AGP试验相比，RIE在从现场疫情采集的样本中检测到IBDV特异性抗原的数量显著更多。发现渗出性法氏囊的抗原含量高于出血性法氏囊，因此推荐将其作为诊断传染性法氏囊病的首选材料。该试验还可用于定量商业灭活疫苗中的IBDV特异性抗原。