反向被动血凝试验在传染性法氏囊病诊断中的应用

Reverse passive haemagglutination test in the diagnosis of infectious bursal disease.

作者信息

Nachimuthu K, Raj G D, Thangavelu A, Venkatesan R A

机构信息

Department of Microbiology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, India.

出版信息

Trop Anim Health Prod. 1995 Feb;27(1):43-6. doi: 10.1007/BF02236335.

DOI:10.1007/BF02236335

PMID:7770951

Abstract

The reverse passive haemagglutination test (RPHA) was used to detect infectious bursal disease (IBD) virus antigen in various organs of experimentally infected chickens and field cases. The results of RPHA were compared with those of agar gel immunodiffusion (AGID) and latex agglutination (LAT) tests. IBD antigen was detected in 86.4%, 80.4% and 80.5% of different organs by RHPA, LAT and AGID respectively. Although the differences are not statistically significant, the LAT is recommended because of cost and speed of obtaining results.

摘要

采用反向被动血凝试验（RPHA）检测实验感染鸡和田间病例各器官中的传染性法氏囊病（IBD）病毒抗原。将RPHA的结果与琼脂凝胶免疫扩散试验（AGID）和乳胶凝集试验（LAT）的结果进行比较。RPHA、LAT和AGID分别在86.4%、80.4%和80.5%的不同器官中检测到IBD抗原。尽管差异无统计学意义，但由于成本和获得结果的速度，推荐使用LAT。