Cegieła U, Janiec W
Katedra i Zakład Farmakologii Slaska Akademia Medyczna.
Przegl Lek. 2000;57(2):122-6.
Development of neoplastic diseases can lead to various metabolic changes in the bone tissue. The aim of the present study was to investigate effects of two antineoplastic agents: amsacrine and cytarabine on the bone system in rats. The experiments were carried out on male Wistar rats (initial body mass: 115-150 g). Amsacrine or cytarabine were administered subcutaneously in a dose of 3 mg/kg daily for four weeks. Cytarabine was administered every day, whereas amsacrine was administered every day for the first 7 days and then, after a five-day break, every second day up to the end of the experiment. The results were compared with those of appropriate control groups. Bone mass, length, diameter, mineral and calcium content, incorporation and release of 45Ca+2 in bones, transverse growth, width of endosteal and periosteal osteoid, transverse cross-section area of the cortical part of the diaphysis and of the marrow cavity in the tibia, width of epiphyseal cartilage, width of trabeculae and mechanical properties in the femur were examined. Administration of amsacrine caused osteopenic changes in rat bones, with decreases in bone mass, mineral content, longitudinal and transverse growth of the long bones, width of endosteal and periosteal osteoid, transverse cross-section area of the cortical part of the tibial diaphysis, width of trabeculae and width of epiphyseal cartilage. Disorders in the calcium metabolism in the bone tissue (decreases in the incorporation and release of 45Ca+2 in bones) and worsening of mechanical properties of the long bones were observed. After administration of cytarabine, the damages to processes of bone tissue remodeling were much lesser. Decreases in bone mass, bone mineral content and incorporation and release of 45Ca+2 were observed.
肿瘤性疾病的发展可导致骨组织发生各种代谢变化。本研究的目的是调查两种抗肿瘤药物：安吖啶和阿糖胞苷对大鼠骨骼系统的影响。实验选用雄性Wistar大鼠（初始体重：115 - 150克）。安吖啶或阿糖胞苷以每日3毫克/千克的剂量皮下注射，持续四周。阿糖胞苷每日给药，而安吖啶在实验的前7天每日给药，然后在停药5天后，每隔一天给药直至实验结束。将结果与相应对照组的结果进行比较。检测了骨质量、长度、直径、矿物质和钙含量、骨中45Ca+2的掺入和释放、横向生长、骨内膜和骨膜类骨质宽度、胫骨骨干皮质部分和骨髓腔的横截面积、骨骺软骨宽度、小梁宽度以及股骨的力学性能。安吖啶给药导致大鼠骨骼出现骨质减少变化，骨质量、矿物质含量、长骨的纵向和横向生长、骨内膜和骨膜类骨质宽度、胫骨骨干皮质部分的横截面积、小梁宽度和骨骺软骨宽度均降低。观察到骨组织钙代谢紊乱（骨中45Ca+2的掺入和释放减少）以及长骨力学性能恶化。阿糖胞苷给药后，对骨组织重塑过程的损害要小得多。观察到骨质量、骨矿物质含量以及45Ca+2的掺入和释放均减少。
