Cohen J, Werrett D J
J Reprod Fertil. 1975 Feb;42(2):301-10. doi: 10.1530/jrf.0.0420301.
Rabbit and mouse spermatozoa from male and female tracts have been examined for their species-antigenic surface character, and for adherent antibodies, by double immunofluorescence techniques. Mouse spermatozoa from the ductus deferens showed an area over the acrosome which was positive to anti-mouse serum that had been absorbed with some male mouse somatic tissues including blood, but those from the uterus and oviduct were not stained. Spermatozoa from the uterus were shown to have an antibody coat on the acrosome, with anti-mouse IgG, but those from the ductus deferens and oviduct did not. Rabbit spermatozoa were more variable but their activity was similar: ejaculated spermatozoa sometimes already had antibody of male origin; the majority of the spermatozoa arriving early in the uterus were coated, but in general those that attained the oviducts were not coated. The results are interpreted as evidence for selection by the female tract of a small antigenically different population; the majority of spermatozoa are rejected and/or destroyed.
已采用双重免疫荧光技术，对来自雄性和雌性生殖道的兔和小鼠精子进行了检查，以确定其物种抗原表面特征以及附着抗体情况。来自输精管的小鼠精子，其顶体上方区域对已用包括血液在内的一些雄性小鼠体细胞组织吸收过的抗小鼠血清呈阳性反应，但来自子宫和输卵管的精子则未被染色。已证实，来自子宫的精子在顶体上有抗小鼠IgG抗体包被，但来自输精管和输卵管的精子则没有。兔精子的情况更具变异性，但其活性相似：射出的精子有时已经带有雄性来源的抗体；早期到达子宫的大多数精子有抗体包被，但一般来说，到达输卵管的精子则没有包被。这些结果被解释为雌性生殖道对一小部分抗原性不同的精子群体进行选择的证据；大多数精子被排斥和/或破坏。
