Taragnat C, Berger M, Jean C L
Physiologie Comparée et Endocrinologie, CNRS U.A., Université Blaise Pascal, Aubiere, France.
J Androl. 1990 May-Jun;11(3):279-86.
The ductus deferens of the mouse contains a major protein with a molecular weight of 34.5 kd called mouse vas deferens protein (MVDP). Immunofluorescence histochemistry and immunoblotting with monoclonal antibodies have been used to investigate the localization, tissue, and species distribution, androgen-regulation, and developmental expression of this protein. Consistent positive immunoreaction was achieved in the ductus deferens epithelium, and immunofluorescence revealed that spermatozoa from the deferent duct were coated with MVDP. Western blot analysis showed the organ specificity of MVDP, which could not be detected in several organs in the mouse. Furthermore, MVDP appeared to be species specific since the proteins extracted from the ductus deferens of man, rat, guinea-pig, rabbit, and hamster did not react with the anti-MVDP probe. MVDP, whose expression is regulated by testosterone, was detectable at 20 days of age and its concentration increased rapidly from 20-30 days.
小鼠的输精管含有一种分子量为34.5kd的主要蛋白质，称为小鼠输精管蛋白（MVDP）。已使用免疫荧光组织化学和单克隆抗体免疫印迹法来研究该蛋白的定位、组织和物种分布、雄激素调节以及发育表达。在输精管上皮中获得了一致的阳性免疫反应，免疫荧光显示来自输精管的精子被MVDP覆盖。蛋白质印迹分析显示了MVDP的器官特异性，在小鼠的多个器官中均未检测到。此外，MVDP似乎具有物种特异性，因为从人、大鼠、豚鼠、兔和仓鼠的输精管中提取的蛋白质与抗MVDP探针不发生反应。MVDP的表达受睾酮调节，在20日龄时可检测到，其浓度在20至30天迅速增加。
