Fukunaga Y, Wada R, Sugita S, Fujita Y, Nambo Y, Imagawa H, Kanemaru T, Kamada M, Komatsu N, Akashi H
Epizootic Research Station, Equine Research Institute, Japan Racing Association, Kokubunji, Tochigi.
J Vet Med Sci. 2000 Jun;62(6):643-6. doi: 10.1292/jvms.62.643.
Equine arteritis virus (EAV) was readily isolated in RK-13 cell monolayers by plaque assay from seminal plasma of experimental carrier stallions when they contained high titers of virus regardless of the presence of non-viral cytotoxicity in the seminal plasma. The cytotoxicity interfered with virus isolation from seminal plasma which contained virus at titers less than 10 PFU/ml. However, it was possible to detect the virus in seminal plasma pretreated with PEG (#6000). EAV was consistently identified by RT-PCR from crude seminal plasma which contained virus at titers of more than 10(2.7) PFU/ml. In vitro detection of EAV by virus isolation supplemented with RT-PCR using seminal plasma was proved to be an effective alternative to the standard test mating as a diagnostic method for carrier stallions.
当实验性携带病毒的种马精液血浆中含有高滴度病毒时，无论精液血浆中是否存在非病毒细胞毒性，均可通过空斑试验在RK - 13细胞单层中轻松分离出马动脉炎病毒（EAV）。这种细胞毒性会干扰从病毒滴度低于10 PFU/ml的精液血浆中分离病毒。然而，有可能在经聚乙二醇（#6000）预处理的精液血浆中检测到病毒。通过RT-PCR从病毒滴度超过10(2.7) PFU/ml的粗精液血浆中始终能鉴定出EAV。事实证明，使用精液血浆通过病毒分离并辅以RT-PCR对EAV进行体外检测，作为种马携带者的诊断方法，是标准试配的一种有效替代方法。
