Sugimura T, Murakami Y, Ogawa T
Department of Veterinary Microbiology, Faculty of Agriculture, Kagoshima University, Japan.
J Vet Med Sci. 2000 Jun;62(6):659-60. doi: 10.1292/jvms.62.659.
The susceptibilities of culture cells to twelve avian influenza virus strains were determined with ten established cell lines including MDCK and ESK cells and three primary culture cells. The established cell lines derived from embryonic swine kidney (ESK) and chicken kidney (CK) primary culture cells were more sensitive to the avian influenza viruses than the other eleven cells. The ESK cell had a particularly higher infective titer than the MDCK cell with and without trypsin supplement in culture medium, and dispersion of the infective titers was narrower than that of the MDCK cell. The ESK cell is a suitable candidate for routine work on avian influenza viruses in laboratories.
利用包括MDCK和ESK细胞在内的10种已建立的细胞系以及3种原代培养细胞，测定了培养细胞对12种禽流感病毒株的敏感性。源自胚胎猪肾（ESK）和鸡肾（CK）原代培养细胞的已建立细胞系比其他11种细胞对禽流感病毒更敏感。在培养基中添加或不添加胰蛋白酶的情况下，ESK细胞的感染滴度均特别高于MDCK细胞，且感染滴度的离散度比MDCK细胞窄。ESK细胞是实验室进行禽流感病毒常规工作的合适候选细胞。
