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本文引用的文献

1
Control of CD4 gene expression: connecting signals to outcomes in T cell development.
Braz J Med Biol Res. 1999 Jul;32(7):785-803. doi: 10.1590/s0100-879x1999000700001.
2
Altered spacing of promoter elements due to the dodecamer repeat expansion contributes to reduced expression of the cystatin B gene in EPM1.由于十二聚体重复序列扩增导致启动子元件间距改变，从而致使EPM1中胱抑素B基因的表达降低。
Hum Mol Genet. 1999 Sep;8(9):1791-8. doi: 10.1093/hmg/8.9.1791.
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Subclass-specific nuclear localization of a novel CD4 silencer binding factor.
J Exp Med. 1999 Jul 19;190(2):281-91. doi: 10.1084/jem.190.2.281.
4
The myb gene family in cell growth, differentiation and apoptosis.细胞生长、分化和凋亡过程中的myb基因家族
Oncogene. 1999 May 13;18(19):3017-33. doi: 10.1038/sj.onc.1202839.
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A potential role for Elf-1 in CD4 promoter function.
J Biol Chem. 1999 Jun 4;274(23):16126-34. doi: 10.1074/jbc.274.23.16126.
6
c-Myb is essential for early T cell development.c-Myb对早期T细胞发育至关重要。
Genes Dev. 1999 May 1;13(9):1073-8. doi: 10.1101/gad.13.9.1073.
7
The notch pathway intermediate HES-1 silences CD4 gene expression.Notch信号通路中间体HES-1使CD4基因表达沉默。
Mol Cell Biol. 1998 Dec;18(12):7166-75. doi: 10.1128/MCB.18.12.7166.
8
Deletional and mutational analyses of the human CD4 gene promoter: characterization of a minimal tissue-specific promoter.
Biochim Biophys Acta. 1998 Nov 8;1442(2-3):109-19. doi: 10.1016/s0167-4781(98)00154-7.
9
CD4 promoter transactivation by human herpesvirus 6.人疱疹病毒6对CD4启动子的反式激活作用
J Virol. 1998 Nov;72(11):8797-805. doi: 10.1128/JVI.72.11.8797-8805.1998.
10
Following antigen challenge, T cells up-regulate cell surface expression of CD4 in vitro and in vivo.抗原刺激后，T细胞在体外和体内均会上调CD4的细胞表面表达。
J Immunol. 1998 Jul 15;161(2):714-20.

小鼠CD4启动子功能需要因子结合位点的精确排列。

Precise arrangement of factor-binding sites is required for murine CD4 promoter function.

作者信息

Sarafova S, Siu G

机构信息

Department of Microbiology and the Integrated Program in Cellular, Molecular and Biophysical Studies, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA.

出版信息

Nucleic Acids Res. 2000 Jul 15;28(14):2664-71. doi: 10.1093/nar/28.14.2664.

DOI:10.1093/nar/28.14.2664
PMID:10908321
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC102665/
Abstract

The control of CD4 expression is linked to the signaling events that mediate T-cell development and is directly dependent on the CD4 promoter. The CD4 promoter does not contain functionally redundant sites: all four factor-binding sites must be intact to achieve wild-type activity. Here we demonstrate that the precise position of three factor-binding sites relative to each other is essential for promoter activity, indicating that they function together as an inseparable cassette for assembly of the transcription initiation complex. Small changes in either phasing or distance between any two sites in this cassette leads to complete abrogation of promoter function. In addition, we demonstrate that one of the factors that bind the promoter cassette is not present in CD8 SP T(C) cells. Thus, this factor is a candidate for mediating the relative subclass specificity of CD4 promoter function in activated CD4 SP T(H) cells.

摘要

CD4表达的调控与介导T细胞发育的信号转导事件相关联，并且直接依赖于CD4启动子。CD4启动子不包含功能冗余位点：所有四个因子结合位点都必须完整才能实现野生型活性。在此我们证明，三个因子结合位点相对于彼此的精确位置对于启动子活性至关重要，这表明它们作为转录起始复合物组装的不可分割的盒式结构共同发挥作用。该盒式结构中任意两个位点之间的相位或距离的微小变化都会导致启动子功能完全丧失。此外，我们证明结合启动子盒式结构的因子之一在CD8 SP T(C)细胞中不存在。因此，该因子是介导活化的CD4 SP T(H)细胞中CD4启动子功能相对亚类特异性的候选因子。