Matveeva O V, Tsodikov A D, Giddings M, Freier S M, Wyatt J R, Spiridonov A N, Shabalina S A, Gesteland R F, Atkins J F
Department of Human Genetics, University of Utah, 15N 2030E Room 7410, Salt Lake City, UT 84112-5330, USA.
Nucleic Acids Res. 2000 Aug 1;28(15):2862-5. doi: 10.1093/nar/28.15.2862.
Design of antisense oligonucleotides targeting any mRNA can be much more efficient when several activity-enhancing motifs are included and activity-decreasing motifs are avoided. This conclusion was made after statistical analysis of data collected from >1000 experiments with phosphorothioate-modified oligonucleotides. Highly significant positive correlation between the presence of motifs CCAC, TCCC, ACTC, GCCA and CTCT in the oligonucleotide and its antisense efficiency was demonstrated. In addition, negative correlation was revealed for the motifs GGGG, ACTG, AAA and TAA. It was found that the likelihood of activity of an oligonucleotide against a desired mRNA target is sequence motif content dependent.
当包含几个活性增强基序并避免活性降低基序时，靶向任何mRNA的反义寡核苷酸的设计效率会更高。这一结论是在对从1000多个硫代磷酸酯修饰的寡核苷酸实验中收集的数据进行统计分析后得出的。结果表明，寡核苷酸中CCAC、TCCC、ACTC、GCCA和CTCT基序的存在与其反义效率之间存在高度显著的正相关。此外，还发现了GGGG、ACTG、AAA和TAA基序的负相关。研究发现，寡核苷酸针对所需mRNA靶标的活性可能性取决于序列基序含量。
