自身整合屏障因子（BAF）在离散的高阶核蛋白复合物中连接DNA。
Barrier-to-autointegration factor (BAF) bridges DNA in a discrete, higher-order nucleoprotein complex.
作者信息
Zheng R, Ghirlando R, Lee M S, Mizuuchi K, Krause M, Craigie R
机构信息
Laboratory of Molecular Biology, National Institute of Diabetes, and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8997-9002. doi: 10.1073/pnas.150240197.
Abstract
Barrier-to-autointegration factor (BAF) is a highly conserved cellular protein that was identified by its activity in protecting retroviral DNA against autointegration. We show that BAF has the property of bridging double-stranded DNA in a highly ordered nucleoprotein complex. Whereas BAF protein alone is a dimer in solution, upon binding DNA, BAF forms a dodecamer with DNA bound at multiple discrete sites in the complex. The interactions between BAF and DNA are entirely nonspecific with respect to DNA sequence. The dual interaction of BAF with DNA and LAP2, a protein associated with the nuclear lamina, suggests a role for LAP2 in chromosome organization. Consistent with this idea, RNA interference experiments with Caenorhabditis elegans reveal a defect in mitosis.
摘要
自身整合屏障因子（BAF）是一种高度保守的细胞蛋白，它通过保护逆转录病毒DNA免受自身整合的活性而被鉴定出来。我们发现BAF具有在高度有序的核蛋白复合物中桥连双链DNA的特性。虽然单独的BAF蛋白在溶液中是二聚体，但在结合DNA后，BAF会形成一个十二聚体，DNA在复合物中的多个离散位点结合。BAF与DNA之间的相互作用在DNA序列方面完全是非特异性的。BAF与DNA以及与核纤层相关的蛋白LAP2之间的双重相互作用表明LAP2在染色体组织中发挥作用。与这一观点一致，秀丽隐杆线虫的RNA干扰实验揭示了有丝分裂中的缺陷。
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本文引用的文献
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Structural basis of DNA bridging by barrier-to-autointegration factor.屏障自整合因子介导DNA桥联的结构基础
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LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction.LAP2结合蛋白1（L2BP1/BAF）是LAP2与染色质相互作用的候选介质。
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Roles of LAP2 proteins in nuclear assembly and DNA replication: truncated LAP2beta proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts.LAP2蛋白在核组装和DNA复制中的作用：截短的LAP2β蛋白改变非洲爪蟾提取物中的核纤层组装、核膜形成、细胞核大小及DNA复制效率。
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The barrier-to-autointegration protein is a host factor for HIV type 1 integration.自身整合屏障蛋白是1型人类免疫缺陷病毒整合的宿主因子。
Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15270-4. doi: 10.1073/pnas.95.26.15270.
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Solution structure of the cellular factor BAF responsible for protecting retroviral DNA from autointegration.负责保护逆转录病毒DNA免受自身整合影响的细胞因子BAF的溶液结构。
Nat Struct Biol. 1998 Oct;5(10):903-9. doi: 10.1038/2345.
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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.双链RNA在秀丽隐杆线虫中产生强大而特异的基因干扰作用。
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A previously unidentified host protein protects retroviral DNA from autointegration.一种先前未被识别的宿主蛋白可保护逆转录病毒DNA免于自身整合。
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