Krisfalusi M, Nagler J J
Center for Reproductive Biology, Department of Biological Sciences, University of Idaho, Moscow 83844-3051, USA.
Mol Reprod Dev. 2000 Aug;56(4):495-501. doi: 10.1002/1098-2795(200008)56:4<495::AID-MRD7>3.0.CO;2-E.
This investigation was conducted to determine the initial period of gonadal sensitivity to estrogen in genetically male rainbow trout (Oncorhynchus mykiss). Fish were immersed in approximately 250 microg estradiol-17beta, (E2)/l for two 2 hr periods during different stages of embryonic development beginning 30 days postfertilization (DPF) and continuing until 68 DPF. Histological analysis of gonad samples indicated a significant proportion of E2-treated fish had intersex gonads; these gonads were primarily comprised of testicular tissue with one or more oocytes scattered throughout. The most sensitive period for altering normal testicular development was found to occur between 44 and 51 DPF (63% intersex), while the labile period in general was determined to span 24 days (from 30 to 54 DPF). Additionally, quantitative RT-PCR was used to measure estrogen receptor (ER) mRNA expression in whole individual untreated embryos at six weekly time points throughout the period of E2-exposure. Although the intersex condition was not observed throughout the entire E2-exposure period, ER mRNA was detected at each time point assayed.
本研究旨在确定遗传雄性虹鳟（Oncorhynchus mykiss）性腺对雌激素的初始敏感时期。在受精后30天（DPF）开始直至68 DPF的胚胎发育不同阶段，将鱼置于约250微克雌二醇-17β（E2）/升的溶液中浸泡两个2小时时段。性腺样本的组织学分析表明，相当比例的经E2处理的鱼具有雌雄同体性腺；这些性腺主要由睾丸组织组成，其间散布着一个或多个卵母细胞。发现改变正常睾丸发育的最敏感时期发生在44至51 DPF之间（63%为雌雄同体），而一般不稳定期确定为24天（从30至54 DPF）。此外，在整个E2暴露期间的六个每周时间点，使用定量RT-PCR测量未处理的单个胚胎整体中的雌激素受体（ER）mRNA表达。尽管在整个E2暴露期间未观察到雌雄同体情况，但在每个检测时间点均检测到了ER mRNA。
