Hervonen H, Eränkö O
Cell Tissue Res. 1975;156(2):145-66. doi: 10.1007/BF00221799.
Lumbar sympathetic ganglia of 12-day-old chick embryos were cultured in organ cultures for 14 days with 1, 10 or 100 mg/l of hydrocortisone or without it. Catecholamines were demonstrated by the formaldehyde-induced fluorescence method. For electron microscopy, the cultures were fixed with glutarialdehyde and osmium tetroxide. Two types of cells with catecholamine fluoresecence were observed in the control cultures: (1) weakly fluorescent sympathetic neurons and sympathicoblasts with long nerve fibres, which were the most common cell type in the explant, and (2) brightly fluorescent cells with or without fluorescent processes, which were less common and were scattered in the explant. Hydrocortisone caused a great increase in the number of the brightly fluorescent cells. With 10 mg/l of hydrocortisone the increase was about ten-fold as compared with the control cultures. There was no change in the morphology of the cells, nor could any change be observed in the fluorescence intensity by eye. Electron microscopically the mature neurons were the most common cell type on the surface of the culture, while more immature sympathicoblasts were seen in the deeper layers. Cells were also found which contained large numbers of catecholamine-strong granular vesicles 105-275 nm in diameter. These cells were infrequent. They had round vesicular nuclei and resembled also in other respects sympathicoblasts or young nerve cells. One such cell was found in mitotic division by electron microscopy. Hydrocortisone caused a marked increase in the number of these granule-containing cells and their processes. Cells which could have been classified as the small intensely fluorescent cells of the mammalian ganglion type or their electron microscopic equivalent, the granule-containing cells were found neither in the control cultures nor in the hydrocortisone-containing cultures. It is concluded that most brightly fluorescent cells in cultured sympathetic ganglia of the chick are nerve cells or sympathicoblasts rich in amine-storing granular vesicles.
将12日龄鸡胚的腰交感神经节在器官培养中培养14天，分别添加1、10或100mg/l氢化可的松，或不添加氢化可的松。采用甲醛诱导荧光法检测儿茶酚胺。用于电子显微镜观察的培养物用戊二醛和四氧化锇固定。在对照培养物中观察到两种具有儿茶酚胺荧光的细胞：（1）荧光较弱的交感神经元和成神经细胞，具有长神经纤维，是外植体中最常见的细胞类型；（2）有或无荧光突起的强荧光细胞，较少见，散在于外植体中。氢化可的松使强荧光细胞数量大幅增加。与对照培养物相比，添加10mg/l氢化可的松时增加约10倍。细胞形态无变化，肉眼也未观察到荧光强度有任何变化。电子显微镜观察显示，成熟神经元是培养物表面最常见的细胞类型，而较不成熟的成神经细胞见于更深层。还发现了一些细胞，其含有大量直径为105 - 275nm的儿茶酚胺强颗粒小泡。这些细胞很少见。它们有圆形泡状核，在其他方面也类似于成神经细胞或年轻神经细胞。通过电子显微镜观察到一个这样的细胞处于有丝分裂期。氢化可的松使这些含颗粒细胞及其突起的数量显著增加。无论是在对照培养物还是含氢化可的松的培养物中，都未发现可归类为哺乳动物神经节类型的小而强荧光细胞或其电子显微镜等效物，即含颗粒细胞。结论是，鸡胚培养交感神经节中大多数强荧光细胞是富含胺储存颗粒小泡的神经细胞或成神经细胞。
