Dias J C, Rezende R P, Rosa C A, Lachance M A, Linardi V R
Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Can J Microbiol. 2000 Jun;46(6):525-31. doi: 10.1139/w00-027.
Candida guilliermondii UFMG-Y65, isolated from a gold mine, was able to utilize different nitriles and the corresponding amides as sole source of nitrogen, at concentrations up to 2 M. Resting cells cultivated on YCB-acetonitrile medium showed nitrile hydrolyzing enzyme activities against acrylonitrile and benzonitrile. These enzymes were inducible and intracellular; the optimum pH was 7.0-8.0, and the optimum temperature 25 degrees C-30 degrees C. Liquid chromatographic analysis indicated that C. guilliermondii UFMG-Y65 metabolized 12 mM benzonitrile to 11 mM benzoic acid and 10 mM acrylonitrile to 7.9 mM acrylic acid. The results suggest that C. guilliermondii UFMG-Y65 may be useful for the bioproduction of amides and acids, and for the bioremediation of environments contaminated with nitriles.
从金矿分离出的季也蒙念珠菌UFMG - Y65能够利用不同的腈类和相应的酰胺作为唯一氮源，浓度可达2M。在YCB - 乙腈培养基上培养的静息细胞对丙烯腈和苯甲腈表现出腈水解酶活性。这些酶是诱导型且存在于细胞内；最适pH为7.0 - 8.0，最适温度为25℃ - 30℃。液相色谱分析表明，季也蒙念珠菌UFMG - Y65将12 mM苯甲腈代谢为11 mM苯甲酸，将10 mM丙烯腈代谢为7.9 mM丙烯酸。结果表明，季也蒙念珠菌UFMG - Y65可能有助于酰胺和酸的生物生产，以及被腈类污染环境的生物修复。
