Rezende R P, Dias J C, Ferraz V, Linardi V R
Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, C.P. 486, 31270-901 Belo Horizonte, Brazil.
J Basic Microbiol. 2000;40(5-6):389-92. doi: 10.1002/1521-4028(200012)40:5/6<389::aid-jobm389>3.0.co;2-j.
The yeast Cryptococcus sp. UFMG-Y28 can utilize benzonitrile as a nitrogen and possible additional carbon source. The kinetics of growth on Yeast Carbon Base (YCB) added of benzonitrile as sole nitrogen source showed that benzonitrile was metabolized to benzoic acid and ammonia. Liquid chromatography analysis indicated that Cryptococcus sp. UFMG-Y28 metabolized 12 mM benzonitrile to 10 mM benzoic acid. Resting cells cultivated on YCB-propionitrile medium showed nitrilase activity against benzonitrile. This strain appears to be promising for bioconversion of nitriles to high value acids and for bioremediation of sites contaminated with aliphatic and aromatic nitriles.
酵母隐球菌属UFMG-Y28能够利用苯甲腈作为氮源以及可能的额外碳源。以添加苯甲腈作为唯一氮源的酵母碳基（YCB）培养基上的生长动力学表明，苯甲腈被代谢为苯甲酸和氨。液相色谱分析表明，隐球菌属UFMG-Y28将12 mM苯甲腈代谢为10 mM苯甲酸。在YCB-丙腈培养基上培养的静息细胞对苯甲腈表现出腈水解酶活性。该菌株在腈生物转化为高价值酸以及被脂肪族和芳香族腈污染场地的生物修复方面似乎很有前景。
