• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Measurement of ceramide levels by the diacylglycerol kinase reaction and by high-performance liquid chromatography-fluorescence spectrometry.

作者信息

Bose R, Kolesnick R

机构信息

School of Medicine at Washington, University Medical Center, Washington University, St. Louis, Missouri 63110, USA.

出版信息

Methods Enzymol. 2000;322:373-8. doi: 10.1016/s0076-6879(00)22034-x.

DOI:10.1016/s0076-6879(00)22034-x
PMID:10914031
Abstract
摘要

相似文献

1
Measurement of ceramide levels by the diacylglycerol kinase reaction and by high-performance liquid chromatography-fluorescence spectrometry.
Methods Enzymol. 2000;322:373-8. doi: 10.1016/s0076-6879(00)22034-x.
2
Quantitative determination of ceramide using diglyceride kinase.使用甘油二酯激酶对神经酰胺进行定量测定。
Methods Enzymol. 2000;312:22-31. doi: 10.1016/s0076-6879(00)12897-6.
3
Current methods for the identification and quantitation of ceramides: an overview.神经酰胺鉴定和定量的当前方法：综述
Lipids. 2000 Sep;35(9):937-45. doi: 10.1007/s11745-000-0603-1.
4
Observation of different ceramide species from crude cellular extracts by normal-phase high-performance liquid chromatography coupled to atmospheric pressure chemical ionization mass spectrometry.通过正相高效液相色谱-大气压化学电离质谱联用技术观察粗细胞提取物中不同种类的神经酰胺。
Rapid Commun Mass Spectrom. 2003;17(11):1203-11. doi: 10.1002/rcm.1043.
5
Analysis of sphingomyelin and ceramide levels and the enzymes regulating their metabolism in response to cell stress.分析鞘磷脂和神经酰胺水平以及响应细胞应激时调节其代谢的酶。
Methods Cell Biol. 2001;66:135-65. doi: 10.1016/s0091-679x(01)66007-2.
6
A specific ceramide kinase assay to measure cellular levels of ceramide.
Anal Biochem. 2003 Sep 15;320(2):259-65. doi: 10.1016/s0003-2697(03)00388-9.
7
A direct method for the simultaneous measurement of ceramide and phospholipase D activity.
Prostaglandins Leukot Essent Fatty Acids. 2000 Oct;63(4):187-94. doi: 10.1054/plef.2000.0205.
8
Ceramide quantitation: evaluation of a mixed micellar assay using E. coli diacylglycerol kinase.
Biochem Mol Biol Int. 1995 May;36(1):21-30.
9
Quantitative measurement of sn-1,2-diacylglycerols.sn-1,2-二酰基甘油的定量测定
Methods Enzymol. 1987;141:294-300. doi: 10.1016/0076-6879(87)41077-x.
10
Preparation and reconstitution of F1F0 and F0 from Escherichia coli.大肠杆菌F1F0和F0的制备与复性。
Methods Enzymol. 1986;126:579-88. doi: 10.1016/s0076-6879(86)26060-7.

引用本文的文献

1
DHA and 19,20-EDP induce lysosomal-proteolytic-dependent cytotoxicity through de novo ceramide production in H9c2 cells with a glycolytic profile.DHA和19,20-环氧二十碳三烯酸（19,20-EDP）通过从头合成神经酰胺，在具有糖酵解特征的H9c2细胞中诱导溶酶体蛋白水解依赖性细胞毒性。
Cell Death Discov. 2018 Aug 20;4:29. doi: 10.1038/s41420-018-0090-1. eCollection 2018.
2
PPARδ signaling mediates the cytotoxicity of DHA in H9c2 cells.过氧化物酶体增殖物激活受体δ（PPARδ）信号传导介导二十二碳六烯酸（DHA）对H9c2细胞的细胞毒性。
Toxicol Lett. 2015 Jan 5;232(1):10-20. doi: 10.1016/j.toxlet.2014.09.029. Epub 2014 Oct 6.
3
Mitofusion-2-mediated alleviation of insulin resistance in rats through reduction in lipid intermediate accumulation in skeletal muscle.
线粒体融合蛋白 2 介导的骨骼肌脂质中间产物积累减少改善大鼠胰岛素抵抗
J Biomed Sci. 2013 Jul 1;20(1):45. doi: 10.1186/1423-0127-20-45.
4
Myocardial adipose triglyceride lipase overexpression protects diabetic mice from the development of lipotoxic cardiomyopathy.心肌脂肪甘油三酯脂肪酶过表达可保护糖尿病小鼠免于发生脂毒性心肌病。
Diabetes. 2013 May;62(5):1464-77. doi: 10.2337/db12-0927. Epub 2013 Jan 24.
5
Inhibition of serine palmitoyl transferase I reduces cardiac ceramide levels and increases glycolysis rates following diet-induced insulin resistance.抑制丝氨酸棕榈酰转移酶 I 可降低饮食诱导的胰岛素抵抗后心脏神经酰胺水平并增加糖酵解速率。
PLoS One. 2012;7(5):e37703. doi: 10.1371/journal.pone.0037703. Epub 2012 May 22.
6
Alpha particles induce apoptosis through the sphingomyelin pathway.α 粒子通过神经酰胺通路诱导细胞凋亡。
Radiat Res. 2011 Oct;176(4):434-46. doi: 10.1667/rr2472.1. Epub 2011 Jun 1.
7
Gamma-tocotrienol induces apoptosis and autophagy in prostate cancer cells by increasing intracellular dihydrosphingosine and dihydroceramide.γ-生育三烯酚通过增加细胞内二氢神经酰胺和二氢鞘氨醇诱导前列腺癌细胞凋亡和自噬。
Int J Cancer. 2012 Feb 1;130(3):685-93. doi: 10.1002/ijc.26054. Epub 2011 May 9.
8
Ceramide synthases 2, 5, and 6 confer distinct roles in radiation-induced apoptosis in HeLa cells.鞘氨醇合酶 2、5 和 6 在 HeLa 细胞辐射诱导凋亡中发挥不同作用。
Cell Signal. 2010 Sep;22(9):1300-7. doi: 10.1016/j.cellsig.2010.04.006. Epub 2010 Apr 18.
9
gamma-Tocopherol or combinations of vitamin E forms induce cell death in human prostate cancer cells by interrupting sphingolipid synthesis.γ-生育酚或维生素E多种形式的组合通过干扰鞘脂合成诱导人前列腺癌细胞死亡。
Proc Natl Acad Sci U S A. 2004 Dec 21;101(51):17825-30. doi: 10.1073/pnas.0408340102. Epub 2004 Dec 13.
10
Signaling mechanisms in tumor necrosis factor alpha-induced death of microvascular endothelial cells of the corpus luteum.肿瘤坏死因子α诱导黄体微血管内皮细胞死亡的信号传导机制
Reprod Biol Endocrinol. 2003 Feb 11;1:17. doi: 10.1186/1477-7827-1-17.