Legallais C, Doré E, Paullier P
Technological University of Compiègne, Department of Biological Engineering, Compiègne Cedex, France.
Artif Organs. 2000 Jul;24(7):519-25. doi: 10.1046/j.1525-1594.2000.06510.x.
The aim of this study was to design a bioreactor for extracorporeal liver supply containing alginate beads in a fluidized bed regimen. The objective was to achieve a satisfactory mixing into the bioreactor to promote the potential exchanges and mass transfers. First, we checked whether both present phases (solid: alginate beads; liquid: saline solution at 20 degrees C) might allow for this fluidization. Then the optimal design was defined as a function of the required operating conditions, bead volume, and perfusion flow rate; the bioreactor cross section and height especially needed to be adjusted. The efficient fluidization, under optimized conditions, was proven through the follow-up of the head losses generated by the fluidized bed. Criteria for scaling up were also determined.
本研究的目的是设计一种用于体外肝脏供应的生物反应器，该反应器在流化床模式下含有藻酸盐珠。目标是在生物反应器中实现令人满意的混合，以促进潜在的交换和传质。首先，我们检查了两个当前相（固体：藻酸盐珠；液体：20摄氏度的盐溶液）是否允许这种流化。然后，根据所需的操作条件、珠体积和灌注流速确定了最佳设计；尤其需要调整生物反应器的横截面和高度。通过跟踪流化床产生的压头损失，证明了在优化条件下的高效流化。还确定了放大标准。
