一种蚜虫内共生菌——布赫纳氏菌中的多胺组成及与多胺生物合成相关基因的表达
Polyamine composition and expression of genes related to polyamine biosynthesis in an aphid endosymbiont, Buchnera.
作者信息
Nakabachi A, Ishikawa H
机构信息
Department of Biological Sciences, Graduate School of Sciences University of Tokyo, Hongo, Japan.
出版信息
Appl Environ Microbiol. 2000 Aug;66(8):3305-9. doi: 10.1128/AEM.66.8.3305-3309.2000.
Abstract
Polyamine composition in an aphid endosymbiotic bacterium, Buchnera sp., was determined by high-performance liquid chromatographic analysis. We found that Buchnera contained virtually only a single polyamine, spermidine. The spermidine content of Buchnera was considerably higher in young aphids and tended to decrease with the age of the host. Expression of speD and speE, whose gene products are key enzymes in the synthesis of spermidine, was analyzed by real-time quantitative reverse transcription-PCR. It was shown that the levels of their mRNAs fluctuated in line with the spermidine content.
摘要
通过高效液相色谱分析确定了一种蚜虫内共生细菌——布赫纳氏菌（Buchnera sp.）中的多胺组成。我们发现布赫纳氏菌实际上只含有一种多胺，即亚精胺。在幼龄蚜虫中，布赫纳氏菌的亚精胺含量相当高，并且往往会随着宿主年龄的增长而降低。通过实时定量逆转录PCR分析了speD和speE的表达，其基因产物是亚精胺合成中的关键酶。结果表明，它们的mRNA水平与亚精胺含量一致波动。
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本文引用的文献
1
Nutritional interactions in insect-microbial symbioses: aphids and their symbiotic bacteria Buchnera.昆虫-微生物共生关系中的营养相互作用：蚜虫及其共生细菌布赫纳氏菌。
Annu Rev Entomol. 1998;43:17-37. doi: 10.1146/annurev.ento.43.1.17.
2
SPERMIDINE, SPERMINE, AND RELATED AMINES.亚精胺、精胺及相关胺类
Pharmacol Rev. 1964 Sep;16:245-300.
3
Provision of riboflavin to the host aphid, Acyrthosiphon pisum, by endosymbiotic bacteria, Buchnera.内共生菌布赫纳氏菌（Buchnera）为宿主蚜虫豌豆蚜（Acyrthosiphon pisum）提供核黄素。
J Insect Physiol. 1999 Jan;45(1):1-6. doi: 10.1016/s0022-1910(98)00104-8.
4
Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS.蚜虫内共生细菌布赫纳氏菌（Buchnera sp.）APS的基因组序列。
Nature. 2000 Sep 7;407(6800):81-6. doi: 10.1038/35024074.
5
Genomic copy number of intracellular bacterial symbionts of aphids varies in response to developmental stage and morph of their host.蚜虫细胞内细菌共生体的基因组拷贝数会因宿主的发育阶段和形态而变化。
Insect Biochem Mol Biol. 2000 Mar;30(3):253-8. doi: 10.1016/s0965-1748(99)00125-3.
6
Intracellular bacterial symbionts of aphids possess many genomic copies per bacterium.蚜虫的细胞内细菌共生体每个细菌拥有许多基因组拷贝。
J Mol Evol. 1999 Jun;48(6):717-22. doi: 10.1007/pl00006516.
7
Ionic and structural specificity effects of natural and synthetic polyamines on the aggregation and resolubilization of single-, double-, and triple-stranded DNA.天然和合成多胺对单链、双链和三链DNA聚集及再溶解的离子和结构特异性效应。
Biochemistry. 1999 Mar 23;38(12):3821-30. doi: 10.1021/bi9825753.
8
Physical and genetic map of the genome of Buchnera, the primary endosymbiont of the pea aphid Acyrthosiphon pisum.豌豆蚜（Acyrthosiphon pisum）主要内共生菌布赫纳氏菌（Buchnera）基因组的物理图谱和遗传图谱。
J Mol Evol. 1999 Feb;48(2):142-50. doi: 10.1007/pl00006452.
9
Differential display of mRNAs related to amino acid metabolism in the endosymbiotic system of aphids.
Insect Biochem Mol Biol. 1997 Dec;27(12):1057-62. doi: 10.1016/s0965-1748(97)00092-1.
10
The complete genome sequence of Escherichia coli K-12.大肠杆菌K-12的全基因组序列。
Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
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