利用高度保守的DNA区域进行PCR以鉴定苜蓿中华根瘤菌。
PCR use of highly conserved DNA regions for identification of Sinorhizobium meliloti.
作者信息
Sánchez-Contreras M, Lloret J, Martín M, Villacieros M, Bonilla I, Rivilla R
机构信息
Departamento de Biología, Universidad Autónoma de Madrid, 28049 Madrid, Spain.
出版信息
Appl Environ Microbiol. 2000 Aug;66(8):3621-3. doi: 10.1128/AEM.66.8.3621-3623.2000.
Abstract
A PCR identification method in which four primers that recognize homologous conserved regions in the Sinorhizobium meliloti genome are used was developed and tested. The regions used for identification were the nodbox 4 locus, which is located in one of the symbiotic megaplasmids, and the mucR gene, which is located in the chromosome. The new method was used to establish a collection of S. meliloti strains from polluted soils.
摘要
开发并测试了一种PCR鉴定方法，该方法使用四种识别苜蓿中华根瘤菌基因组中同源保守区域的引物。用于鉴定的区域是位于一个共生大质粒上的nodbox 4基因座，以及位于染色体上的mucR基因。该新方法用于建立一组来自污染土壤的苜蓿中华根瘤菌菌株。
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