Appl Environ Microbiol. 1995 Oct;61(10):3701-4. doi: 10.1128/aem.61.10.3701-3704.1995.
A halotolerant strain of Rhizobium meliloti was isolated from nodules of a Melilotus plant growing in a salt marsh in Donana National Park (southwest Spain). This strain, EFB1, is able to grow at NaCl concentrations of up to 500 mM, and no effect on growth is produced by 300 mM NaCl. EFB1 showed alterations on its lipopolysaccharide (LPS) structure that can be related to salt stress: (i) silver-stained electrophoretic profiles showed a different mobility that was dependent on ionic stress but not on osmotic pressure, and (ii) a monoclonal antibody, JIM 40, recognized changes in LPS that were dependent on osmotic stress. Both modifications on LPS may form part of the adaptive mechanism of this bacterium for saline environments.
从生长在西班牙西南部多纳纳国家公园（Donana National Park）盐沼中的草木樨属植物根瘤中分离出一株耐盐根瘤菌 Rhizobium meliloti 菌株。该菌株 EFB1 能够在高达 500mM 的 NaCl 浓度下生长，300mM NaCl 对其生长没有影响。EFB1 的脂多糖（LPS）结构发生改变，这可能与盐胁迫有关：（i）银染电泳图谱显示出不同的迁移率，这种迁移率依赖于离子胁迫而不是渗透压，（ii）单克隆抗体 JIM 40 识别出 LPS 的变化，这种变化依赖于渗透压。LPS 的这两种修饰可能是该细菌适应盐环境的适应机制的一部分。
