Summers M C, McGinnis L K, Lawitts J A, Raffin M, Biggers J D
Fertility Center of New England, Reading, MA 01867, USA.
Hum Reprod. 2000 Aug;15(8):1791-801. doi: 10.1093/humrep/15.8.1791.
The addition of amino acids to a modified simplex optimized medium (mKSOM) did not increase the percentage of blastocysts that develop from CF1 mouse ova fertilized in vitro. In contrast, the percentage of blastocysts that began to hatch and the number of cells in these blastocysts, particularly in the inner cell mass, was increased. The added amino acids also supported the development of a more organized extracellular matrix in the same blastocysts. The results suggest that zygotes produced in amino acid-supplemented mKSOM have a greater developmental potential, perhaps developing at a faster rate, than zygotes produced in mKSOM. This enhanced developmental potential may be caused by the alleviation of osmotic stress on the ova and zygotes by the amino acids that are osmolytes. The fertilization of human ova in vitro may benefit from the inclusion of free amino acids in the fertilizing medium. The availability of a medium that can be used to support both IVF and preimplantation development in the mouse is likely to benefit the recovery of mouse strains from cryopreserved spermatozoa.
在改良的单纯形优化培养基（mKSOM）中添加氨基酸，并未提高体外受精的CF1小鼠卵子发育成囊胚的比例。相比之下，开始孵化的囊胚比例以及这些囊胚中的细胞数量，尤其是内细胞团中的细胞数量增加了。添加的氨基酸还促进了同一囊胚中更有序的细胞外基质的形成。结果表明，在添加氨基酸的mKSOM中产生的合子比在mKSOM中产生的合子具有更大的发育潜力，可能发育速度更快。这种增强的发育潜力可能是由于作为渗透剂的氨基酸减轻了卵子和合子的渗透应激所致。体外受精的人类卵子可能会受益于受精培养基中包含游离氨基酸。一种可用于支持小鼠体外受精和植入前发育的培养基的可用性可能有利于从冷冻保存的精子中恢复小鼠品系。
