Zoology Department, University of Oxford, 11a Mansfield Road, Oxford, OX1 3SZ, UK.
Nuffield Department of Women's Reproductive Health, Level 3, Women's Centre, John Radcliffe Hospital, Oxford, OX3 9DU, UK.
Biol Open. 2021 Dec 15;10(12). doi: 10.1242/bio.059013. Epub 2021 Dec 22.
Mouse zygote morphokinetics were measured during interphase, the mitotic period, cytokinesis, and two-cell stage. Sequences of rounder-distorted-rounder shapes were revealed, as were changing patterns of cross section area. A calcium chelator and an actin-disrupting agent inhibited the area changes that occurred between pronuclear envelope breakdown and cytokinesis. During cell division, two vortices developed in each nascent cell and they rotated in opposite directions at each end of the cell, a pattern that sometimes persisted for up to 10 h. Exchange with the environment may have been promoted by these shape and area cycles and persisting circulation in the cytoplasm may have a similar function between a cell's interior and periphery. Some of these movements were sporadically also seen in human zygotes with abnormal numbers of pronuclei and the two-cell stages that developed from these compromised human zygotes.
在间期、有丝分裂期、胞质分裂期和两细胞期测量了小鼠受精卵的形态动力学。揭示了从圆形到变形圆形再到圆形的序列，以及截面积变化的模式。钙螯合剂和肌动蛋白破坏剂抑制了从原核膜破裂到胞质分裂期间发生的面积变化。在细胞分裂过程中，每个新形成的细胞中都形成了两个涡流，它们在细胞的两端以相反的方向旋转，这种模式有时会持续长达 10 小时。这种形状和面积的循环可能促进了与环境的交换，而细胞质中持续的循环可能在细胞内部和外部之间具有类似的功能。在异常数量的原核和由此产生的两细胞期的人类受精卵中也偶尔观察到了其中一些运动。
