Satheeshkumar K, Seeni S
Plant Biotechnology Division, Tropical Botanic Garden and Research Institute, Thiruvananthapuram, India.
Indian J Exp Biol. 2000 Mar;38(3):273-7.
In vitro multiplication of Nothapodites foetida (Wight.) Sleumer was achieved using axenic seedling explant cultures. Isolated nodes (1.0-1.2 cm) and shoot tips (1.0-1.5 cm) cultured in Murashige and Skoog's agar medium containing varying concentrations of TDZ, BA and combinations of 2iP and GA3. Single shoot (0.8-1.2 cm) was regenerated in each culture after 6 weeks. Axillary shoots were then excised and recultured for 8 weeks in medium containing TDZ (0.05 mgL-1) which formed shoots (about 4 in no.; 2 cm) from the basal node. Axillary branches (2) which formed on 60% of these shoots after 10-12 weeks of culture were separated and recultured in the same medium for 8 weeks. Three shoots (0.8-1.0 cm) per culture were regenerated. Shoots of 0.8-1.8 cm length were subcultured on a low cytokinin (0.01 mgL-1 TDZ) regime to induce shoot elongation (2.0-3.5 cm) in 4 weeks. Shoot cuttings were rooted (60%) in the medium containing IBA (1.5 mgL-1). Rooted plantlets established in pots (90%) after hardening resumed normal growth in 3 months.
利用无菌幼苗外植体培养实现了臭豆叶楠（Nothapodites foetida (Wight.) Sleumer）的离体增殖。将分离的节段（1.0 - 1.2厘米）和茎尖（1.0 - 1.5厘米）接种于含有不同浓度噻苯隆（TDZ）、苄氨基嘌呤（BA）以及2 - 异戊烯腺嘌呤（2iP）与赤霉素（GA3）组合的Murashige和Skoog琼脂培养基中。6周后，每个培养物中再生出单茎（0.8 - 1.2厘米）。然后切下腋芽，在含有噻苯隆（0.05毫克/升）的培养基中再培养8周，腋芽从基部节段形成茎（约4个；2厘米）。培养10 - 12周后，60%的这些茎上形成的腋枝（2个）被分离，并在相同培养基中再培养8周。每个培养物再生出三个茎（0.8 - 1.0厘米）。将长度为0.8 - 1.8厘米的茎在低细胞分裂素（0.01毫克/升噻苯隆）条件下继代培养，4周内诱导茎伸长至（2.0 - 3.5厘米）。茎插条在含有吲哚丁酸（IBA，1.5毫克/升）的培养基中生根（60%）。炼苗后，90%在花盆中定植的生根小植株在3个月内恢复正常生长。
