Saini R, Jaiwal P K
Department of Biosciences, M.D. University, Rohtak 124001, India.
Indian J Exp Biol. 2000 May;38(5):499-503.
The frequency of shoot regeneration and multiplication of P. harmala was influenced by the type of explant and kind and concentration of hormones. Of the various seedling explants, cotyledonary node exhibited maximum shoot regeneration frequency from axillary region on MS medium supplemented with 5 microM BAP. Addition of 0.1 microM NAA enhanced the efficacy of BAP for multiple shoot regeneration as well as improved the growth of shoots. BAP (5 microM) in combination with NAA (0.1 microM) was found to be the optimal for inducing an average of 4-5 shoots per explant in 75% of the cultures within 5 weeks. Replacement of BAP with other cytokinins at equimolar concentration of BAP i.e. 5 microM was not effective in inducing multiple shoots. Regenerated shoots were rooted on MS medium containing IBA (8 microM) with 80% efficiency. The plantlets were successfully established in soil where 80% of them developed into morphological normal plants.
骆驼蓬的芽再生频率和增殖受到外植体类型、激素种类和浓度的影响。在各种幼苗外植体中，子叶节在添加5微摩尔/升苄氨基嘌呤（BAP）的MS培养基上，从腋芽区域表现出最高的芽再生频率。添加0.1微摩尔/升萘乙酸（NAA）增强了BAP对多芽再生的效果，并改善了芽的生长。发现BAP（5微摩尔/升）与NAA（0.1微摩尔/升）组合是在5周内75%的培养物中每个外植体平均诱导出4 - 5个芽的最佳组合。用等摩尔浓度（即5微摩尔/升）的其他细胞分裂素替代BAP在诱导多芽方面无效。再生芽在含有8微摩尔/升吲哚丁酸（IBA）的MS培养基上生根，生根效率为80%。这些植株在土壤中成功定植，其中80%发育成形态正常的植株。
