Satoh K, Sakagami H, Terasaka H, Ida Y, Fujisawa S
Analysis Center, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan.
Anticancer Res. 2000 May-Jun;20(3A):1577-81.
The interaction between hydroquinone (HQ) and ascorbic acid analogs was investigated. Semiquinone radicals (SQ.) generated by HQ were comparably scavenged by ascorbic acid, sodium ascorbate and ascorbate 6-palmitate, but much less efficiently by ascorbate 2,6-dipalmitate. Organic solvents, such as dimethysulfoxide (DMSO) or ethanol, which are utilized for solubilization of water-insoluble analogs, ascorbate 6-palmitate and ascorbate 2,6-dipalmitate, also scavenged SQ.. DMSO scavenged the SQ. more efficiently than ethanol. This suggests the importance of considering such a quenching effect of organic solvent in the study of the interaction between HQ and antioxidants. The involvement of the ascorbate radical for cytotoxicity induction is discussed.
研究了对苯二酚（HQ）与抗坏血酸类似物之间的相互作用。由HQ产生的半醌自由基（SQ.）可被抗坏血酸、抗坏血酸钠和抗坏血酸6-棕榈酸酯同等程度地清除，但被抗坏血酸2,6-二棕榈酸酯清除的效率要低得多。用于溶解水不溶性类似物抗坏血酸6-棕榈酸酯和抗坏血酸2,6-二棕榈酸酯的有机溶剂，如二甲亚砜（DMSO）或乙醇，也能清除SQ.。DMSO清除SQ.的效率比乙醇更高。这表明在研究HQ与抗氧化剂之间的相互作用时，考虑有机溶剂的这种淬灭作用很重要。讨论了抗坏血酸自由基在细胞毒性诱导中的作用。
