Hao C F, Wang Z H, Yun J P, Li H K, Ng C C, To K F, Chew-Cheng S B, Chew E C
Department of Anatomy, Chinese University of Hong Kong, Shatin, NT, Hong Kong.
Anticancer Res. 2000 May-Jun;20(3A):1599-602.
Sixty gliomas obtained by neurosurgical resections were examined. Paraffin blocks were retrieved from pathological files of the Second Affiliated Hospital in Guangzhou Medical College. The methods of argyrophilic technique for AgNORs staining, and Image Analysis System for measurement of AgNORs were used. Six parameters, which included hcount, count, narea, agnrea, agpern and agperc were used to correlated well with histopathological grades (compared grade 2 & 3, grade 3 & 4, and grade 2 & 4, respectively). We concluded that AgNORs is useful in evaluating proliferative activity and assessing the malignancy of human gliomas. It may also be used as a target for anti-neoplastic drugs in the treatment of gliomas.
对通过神经外科手术切除获得的60例胶质瘤进行了检查。石蜡块取自广州医学院第二附属医院的病理档案。采用嗜银技术进行AgNORs染色，并使用图像分析系统测量AgNORs。使用hcount、count、narea、agnrea、agpern和agperc这六个参数，分别与组织病理学分级（比较2级与3级、3级与4级以及2级与4级）有良好的相关性。我们得出结论，AgNORs在评估人类胶质瘤的增殖活性和恶性程度方面是有用的。它也可作为胶质瘤治疗中抗肿瘤药物的靶点。
