Ventura M, Callegari M L, Morelli L
Centro Richerche Biotecnologiche, Università Cattolica del Sacro Cuore, Cremona, Italy.
FEMS Microbiol Lett. 2000 Aug 15;189(2):275-9. doi: 10.1111/j.1574-6968.2000.tb09243.x.
The paper describes two methods that allow rapid and accurate identification of Lactobacillus helveticus strains based on the nucleotide sequence of the gene coding for the surface layer (S-layer) protein. The first method is based on a polymerase chain reaction amplification using primers targeting a specific fragment of the S-layer gene. The second method involved a portion of the S-layer gene of L. helveticus as a probe in a hybridisation test. The specificity of these protocols was checked for DNA samples isolated from various Lactobacillus strains.
本文描述了两种基于编码表层（S 层）蛋白的基因核苷酸序列快速准确鉴定瑞士乳杆菌菌株的方法。第一种方法基于聚合酶链反应扩增，使用靶向 S 层基因特定片段的引物。第二种方法是将瑞士乳杆菌 S 层基因的一部分作为探针用于杂交试验。针对从各种乳酸杆菌菌株中分离的 DNA 样本检查了这些方案的特异性。
