Fortina M G, Ricci G, Mora D, Parini C, Manachini P L
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Sezione di Microbiologia Industriale, Università degli Studi di Milano, Via Celoria 2, 20133, Milan, Italy.
FEMS Microbiol Lett. 2001 Apr 20;198(1):85-9. doi: 10.1111/j.1574-6968.2001.tb10623.x.
Specific regions in three genes coding for aminopeptidases C and N, and a trypsin-like serine protease were selected as species-specific primer sequences for rapid and reliable identification of Lactobacillus helveticus strains. The PCR procedures carried out gave specific 524-, 726- and 918-bp amplificates, with DNA isolated from L. helveticus. No PCR product was generated for closely related bacteria. The amplification products were also screened for their species specificity in dot blot hybridization with representatives of the most closely related genera and species and a number of other bacterial species.
