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1
Phenylalanyl-tRNA synthetase from baker's yeast: role of 3'-terminal adenosine of tRNA-Phe in enzyme-substrate interaction studied with 3'-modified tRNA-Phe species.来自面包酵母的苯丙氨酰 - tRNA合成酶：用3' - 修饰的tRNA - Phe物种研究tRNA - Phe的3' - 末端腺苷在酶 - 底物相互作用中的作用。
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1378-82. doi: 10.1073/pnas.72.4.1378.
2
Hydrolytic action of aminoacyl-tRNA synthetases from baker's yeast: "chemical proofreading" preventing acylation of tRNA(I1e) with misactivated valine.面包酵母氨酰 - tRNA合成酶的水解作用：“化学校对”可防止tRNA(I1e)被错误激活的缬氨酸酰化。
Biochemistry. 1976 Sep 7;15(18):4131-8. doi: 10.1021/bi00663a034.
3
Modification of phenylalanyl-tRNA synthetase from baker's yeast by proteolytic cleavage and properties of the trypsin-modified enzyme.通过蛋白水解切割对面包酵母苯丙氨酰 - tRNA合成酶进行修饰以及胰蛋白酶修饰酶的性质
Eur J Biochem. 1975 May 6;53(2):487-92. doi: 10.1111/j.1432-1033.1975.tb04090.x.
4
Phenylalanyl-tRNA, lysyl-tRNA, isoleucyl-tRNA and arginyl-tRNA synthetases. Substrate specificity in the ATP/PPi exchange with regard to ATP analogs.苯丙氨酰 - 转运RNA、赖氨酰 - 转运RNA、异亮氨酰 - 转运RNA和精氨酰 - 转运RNA合成酶。关于ATP类似物在ATP/焦磷酸交换中的底物特异性。
Eur J Biochem. 1980;107(1):47-50. doi: 10.1111/j.1432-1033.1980.tb04622.x.
5
Phenylalanyl-tRNA synthetase of baker's yeast. Modulation of adenosine triphosphate-pyrophosphate exchange by transfer ribonucleic acid.面包酵母的苯丙氨酰 - tRNA合成酶。转移核糖核酸对三磷酸腺苷 - 焦磷酸交换的调节作用。
Biochemistry. 1981 Jun 23;20(13):3851-6. doi: 10.1021/bi00516a028.
6
On the stereochemistry of activation of phenylalanine by phenylalanyl-tRNA synthetase from baker's yeast.关于面包酵母苯丙氨酰 - tRNA合成酶激活苯丙氨酸的立体化学研究。
Eur J Biochem. 1977 Jun 1;76(1):263-7. doi: 10.1111/j.1432-1033.1977.tb11591.x.
7
Phenylalanyl-tRNA and seryl-tRNA synthetases from baker's yeast. Substrate specificity with regard to ATP analogs and mechanism of the aminoacylation reaction.来自面包酵母的苯丙氨酰 - tRNA合成酶和丝氨酰 - tRNA合成酶。关于ATP类似物的底物特异性及氨酰化反应机制。
Eur J Biochem. 1976 May 1;64(2):389-93. doi: 10.1111/j.1432-1033.1976.tb10313.x.
8
Valyl- and phenylalanyl-tRNA synthetase from baker's yeast: recognition of transfer RNA results from a multistep process, as indicated by inhibition of aminoacylation with modified transfer RNA.来自面包酵母的缬氨酰 - 和苯丙氨酰 - tRNA合成酶：tRNA的识别源于一个多步骤过程，如用修饰的tRNA抑制氨酰化所示。
Biochemistry. 1978 Oct 17;17(21):4509-14. doi: 10.1021/bi00614a023.
9
A novel enzymatic activity of phenylalanyl transfer ribonucleic acid synthetase from baker's yeast: zinc ion induced transfer ribonucleic acid independent hydrolysis of adenosine triphosphate.来自面包酵母的苯丙氨酰转移核糖核酸合成酶的一种新型酶活性：锌离子诱导的不依赖转移核糖核酸的三磷酸腺苷水解。
Biochemistry. 1980 Apr 15;19(8):1676-80. doi: 10.1021/bi00549a024.
10
Prokaryotic and eukaryotic tetrameric phenylalanyl-tRNA synthetases display conservation of the binding mode of the tRNA(Phe) CCA end.原核生物和真核生物的四聚体苯丙氨酰-tRNA合成酶在tRNA（Phe）CCA末端的结合模式上表现出保守性。
Biochemistry. 2003 Sep 16;42(36):10697-708. doi: 10.1021/bi034732q.

引用本文的文献

1
Functional group recognition at the aminoacylation and editing sites of E. coli valyl-tRNA synthetase.大肠杆菌缬氨酰 - tRNA合成酶氨酰化和校对位点的功能基团识别
RNA. 2004 Mar;10(3):493-503. doi: 10.1261/rna.5166704.
2
Transfer RNA determinants for translational editing by Escherichia coli valyl-tRNA synthetase.大肠杆菌缬氨酰-tRNA合成酶进行翻译编辑的转运RNA决定因素。
Nucleic Acids Res. 2002 Jun 1;30(11):2538-45. doi: 10.1093/nar/30.11.2538.
3
Induced hydrolytic activity of yeast phenylalanyl-tRNA synthetase by tRNAPhe-CC.tRNAPhe-CC诱导酵母苯丙氨酰-tRNA合成酶的水解活性
Nucleic Acids Res. 1982 Apr 10;10(7):2439-51. doi: 10.1093/nar/10.7.2439.
4
Site of aminoacylation of tRNAs from Escherichia coli with respect to the 2'- or 3'-hydroxyl group of the terminal adenosine.大肠杆菌中tRNA相对于末端腺苷2'-或3'-羟基的氨酰化位点。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3049-53. doi: 10.1073/pnas.72.8.3049.
5
[Corrective steps in amino acid activation for protein biosynthesis].
Naturwissenschaften. 1976 Nov;63(11):519-22. doi: 10.1007/BF00596848.
6
Mechanism of tRNA-synthetase recognition: role of terminal A.tRNA合成酶识别机制：末端A的作用
Nucleic Acids Res. 1977 Jul;4(7):2253-62. doi: 10.1093/nar/4.7.2253.
7
The effect of tRNA and tryptophanyl adenylate on limited proteolysis of beef pancreas tryptophanyl-tRNA synthetase.转运核糖核酸及色氨酰腺苷酸对牛胰色氨酰转运核糖核酸合成酶有限度蛋白水解作用的影响
Nucleic Acids Res. 1979 Oct 10;7(3):625-37. doi: 10.1093/nar/7.3.625.
8
Properies of tRNAPhe from yeast carrying a spin label on the 3'-terminal. Interaction with yeast phenylalanyl-tRNA Synthetase and elongation factor Tu from Escherichia coli.来自酵母的在3'-末端带有自旋标记的苯丙氨酸转运RNA（tRNAPhe）的特性。与酵母苯丙氨酰-tRNA合成酶和大肠杆菌延伸因子Tu的相互作用。
Nucleic Acids Res. 1978 Mar;5(3):861-77. doi: 10.1093/nar/5.3.861.
9
ATP-PPi exchange activity of progesterone receptor.孕酮受体的ATP-焦磷酸交换活性
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3443-7. doi: 10.1073/pnas.73.10.3443.

本文引用的文献

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Natural configuration of the purine nucleotides in ribonucleic acids; chemical hydrolysis of the dinucleoside phosphates.核糖核酸中嘌呤核苷酸的天然构型；二核苷磷酸的化学水解。
Nature. 1953 Jun 27;171(4365):1151-2. doi: 10.1038/1711151a0.
2
Active complexes derived from Escherichia coli formylmethionine tRNA which lacks the dihydrouridine-containing loop.源自大肠杆菌甲酰甲硫氨酸tRNA的活性复合物，该tRNA缺少含二氢尿苷的环。
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Mischarging in mutant tyrosine transfer RNAs.突变型酪氨酸转运核糖核酸中的错载。
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Reconstitution of alanine acceptor activity from fragments of yeast tRNA-Ala II.从酵母tRNA-丙氨酸II片段重建丙氨酸受体活性
Nature. 1969 Jun 21;222(5199):1147-8. doi: 10.1038/2221147a0.
6
The yeast phenylalanyl-transfer RNA synthetase recognition site: the region adjacent to the dihydrouridine loop.酵母苯丙氨酰 - 转移RNA合成酶识别位点：与二氢尿嘧啶环相邻的区域。
Proc Natl Acad Sci U S A. 1971 Mar;68(3):681-4. doi: 10.1073/pnas.68.3.681.
7
Transfer RNA, II. A structural basis for alanine acceptor activity.转移核糖核酸，II. 丙氨酸受体活性的结构基础。
Proc Natl Acad Sci U S A. 1968 Sep;61(1):308-15. doi: 10.1073/pnas.61.1.308.
8
Isolation and properties of tRNA nucleotidyl transferase from yeast.酵母中tRNA核苷酸转移酶的分离与性质
Eur J Biochem. 1971 Sep 24;22(2):166-72. doi: 10.1111/j.1432-1033.1971.tb01528.x.
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Localization of two recognition sites in yeast valine tRNA I.酵母缬氨酸tRNA I中两个识别位点的定位
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10
Accepting site for aminoacylation of tRNAphe from yeast.来自酵母的tRNAphe氨酰化的接受位点。
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来自面包酵母的苯丙氨酰 - tRNA合成酶：用3' - 修饰的tRNA - Phe物种研究tRNA - Phe的3' - 末端腺苷在酶 - 底物相互作用中的作用。

Phenylalanyl-tRNA synthetase from baker's yeast: role of 3'-terminal adenosine of tRNA-Phe in enzyme-substrate interaction studied with 3'-modified tRNA-Phe species.

作者信息

Von Der Haar F, Gaertner E

出版信息

Proc Natl Acad Sci U S A. 1975 Apr;72(4):1378-82. doi: 10.1073/pnas.72.4.1378.

DOI:10.1073/pnas.72.4.1378
PMID:1093181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432537/
Abstract

TRNA-Phe species from baker's yeast modified at the 3'-terminus in many cases are phenylalanylatable substrates. Out of several tRNA-Phe species possessing a modified 3'-end that cannot be phenylalanylated, only two, tRNA-Phe-C-C-2'dA and the tRNA-Phe-C-C-formycin-oxi-red, are strong competitive inhibitors for tRNA-Phe-C-C-A during phenylalanylation. In the ATP/PPi exchange, both these inhibitors reduce Vmax to about 25%; but whereas tRNA-Phe-C-C-2dA has no influence on KmATP and Km Phe during ATP/PPi exchange, tRNA-Phe-C-C-formycin-oxi-red reduces KmATP from 1430 muM, found in the absence of tRNA-Phe, to 230 muM, and Km-Phe, from 38 to 14 muM. The values found in the presence of tRNA-Phe-C-C-formycin-oxi-red during ATP/PPi exchange are identical with those determined in the phenylalanylation of tRNA-Phe-C-C-A. All other tRNA-Phe species carrying a modified 3'end that cannot be phenylalanylated exhibit a mixed competitive-noncompetitive inhibition in the phenylalanylation reaction. In the ATP/PPi exchange, they do not influence KmATP and KmPHE and only weakly, if at all, Vmax. The results show that the 3'adenosine of tRNA-Phe cannot solely be a passive acceptor for phenylalanine, but must in addition play an active role during enzyme-substrate interaction. The data can be consistently explained by the hypothesis that the 3'-adenosine of tRNA-Phe triggers a conformational change of the enzyme.

摘要

许多情况下，来自面包酵母的3'-末端经修饰的tRNA-Phe物种是可苯丙氨酰化的底物。在几种具有无法进行苯丙氨酰化修饰的3'-末端的tRNA-Phe物种中，只有两种，即tRNA-Phe-C-C-2'dA和tRNA-Phe-C-C-霉酚酸氧化还原型，在苯丙氨酰化过程中是tRNA-Phe-C-C-A的强竞争性抑制剂。在ATP/PPi交换中，这两种抑制剂都将Vmax降低到约25%；但是，虽然tRNA-Phe-C-C-2dA在ATP/PPi交换过程中对KmATP和Km Phe没有影响，但tRNA-Phe-C-C-霉酚酸氧化还原型将KmATP从无tRNA-Phe时的1430 μM降低到230 μM，将Km-Phe从38 μM降低到14 μM。在ATP/PPi交换过程中，在tRNA-Phe-C-C-霉酚酸氧化还原型存在下测得的值与在tRNA-Phe-C-C-A的苯丙氨酰化过程中测得的值相同。所有其他携带无法进行苯丙氨酰化修饰的3'-末端的tRNA-Phe物种在苯丙氨酰化反应中表现出混合竞争性-非竞争性抑制。在ATP/PPi交换中，它们不影响KmATP和KmPHE，并且对Vmax的影响微弱（如果有影响的话）。结果表明，tRNA-Phe的3'-腺苷不能仅仅是苯丙氨酸的被动受体，而且在酶-底物相互作用过程中必须发挥积极作用。这些数据可以通过tRNA-Phe的3'-腺苷触发酶的构象变化这一假设得到一致解释。