Freist W, von der Haar F, Sprinzl M, Cramer F
Eur J Biochem. 1976 May 1;64(2):389-93. doi: 10.1111/j.1432-1033.1976.tb10313.x.
Eighteen analogs of ATP have been tested in the aminoacylation reaction of phenylalanyl-tRNA and seryl-tRNA synthetases from baker's yeast. Four compounds are substrates for phenylalanyl-tRNA synthetase, five for seryl-tRNA synthetase, one compound is an inhibitor for both enzymes; their Km and Ki and V values have been determined. The substrate specificity shows that for the catalytic action of both enzymes with these substrates positions 6, 7, 8 and 9 of the purine moiety and positions 2' and 3' of the ribose moiety are important.
已对18种ATP类似物进行了测试，以研究其在面包酵母苯丙氨酰 - tRNA和丝氨酰 - tRNA合成酶的氨酰化反应中的作用。有4种化合物是苯丙氨酰 - tRNA合成酶的底物，5种是丝氨酰 - tRNA合成酶的底物，1种化合物是这两种酶的抑制剂；已测定了它们的Km、Ki和V值。底物特异性表明，对于这两种酶与这些底物的催化作用，嘌呤部分的6、7、8和9位以及核糖部分的2'和3'位很重要。
