tRNAPhe-CC诱导酵母苯丙氨酰-tRNA合成酶的水解活性
Induced hydrolytic activity of yeast phenylalanyl-tRNA synthetase by tRNAPhe-CC.
作者信息
Kuhn W, Schneider F W
出版信息
Nucleic Acids Res. 1982 Apr 10;10(7):2439-51. doi: 10.1093/nar/10.7.2439.
Abstract
"Induced hydrolysis" a new hydrolytic activity, was found by measuring AMP-production during aminoacylation of tRNAPhe-CCA by yeast phenylalanyl-tRNA synthetase in the presence of tRNAPhe-CC under conditions of low ionic strength at pH 8.5. Experiments using the elongation factor Tu . GTP provide evidence that transfer of phenylalanine to the tRNAPhe-CCA is followed by rapid hydrolysis in the presence of tRNAPhe-CC. A simple mechanism shows good agreement with the experimental data.
摘要
“诱导水解”是一种新的水解活性，它是在pH 8.5的低离子强度条件下，通过测量酵母苯丙氨酰 - tRNA合成酶在tRNAPhe - CC存在时对tRNAPhe - CCA进行氨酰化反应过程中AMP的产生而发现的。使用延伸因子Tu·GTP进行的实验表明，在tRNAPhe - CC存在的情况下，苯丙氨酸转移到tRNAPhe - CCA后会迅速发生水解。一个简单的机制与实验数据吻合良好。
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本文引用的文献
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